Fig 1: Prior exercise and insulin redistributed intramyocellular GLUT4 in humans. Human muscle biopsies from resting and prior exercised leg before and after insulin stimulation were cut in 70 nm sections at cryogenic temperature. Sections were labelled with GLUT4 and various compartment markers, and the GLUT4 content in each compartment was estimated using Mander's overlap coefficient. (A) GLUT4 overlap with vesicle associated membrane protein 2 (VAMP2) in human skeletal muscle. (B) GLUT4 overlap with trans Golgi network protein 2 (TGN46). (C) GLUT4 overlap with lysosome associated membrane protein 1 (LAMP1). (D) GLUT4 overlap with early endosome antigen 1 (EEA1). (E) GLUT4 overlap with sorting nexin 1 (SNX1). (F) GLUT4 overlap with caveolin 3 (Cav3). (G) GLUT4 overlap with dihydropyridine receptor a1 (DHPR) and Sodium–Calcium Exchanger 1 (NCX1). GSV = GLUT4 Storage Vesicles, Early Endo = Early endosomes, Late/Rec. Endo = Late/Recycling endosomes. n= (basal rest; basal exercise; insulin rest; insulin exercise) A)41; 38; 40; 29, B)32; 31; 31; 24, C)25; 30; 29; 22, D)34; 27; 33; 25, E)19; 20; 25; 19, F)61; 52; 55; 49, G)37; 36; 35; 36(DHPR) and 25; 24; 23; 23(NCX1). #/##/###p < 0.05/0.01/0.001 different from resting leg. */**/***p < 0.05/0.01/0.001 effect of insulin stimulation.