Fig 2: Comparison of S100P concentration in each cell line and each xenograft tumor provided by fine needle aspiration. The concentration of the undiluted solution was calculated in accordance with the dilution rate. *Kruskal-Wallis test. †Mann-Whitney U test. ‡Fine needle aspiration samples obtained from xenograft mice. §Cancer-associated fibroblasts. ?hTERT-HPNE E6/E7/K-RasG12D/st human normal pancreatic duct epithelium.

Fig 3: STARD flow diagram of the study. Index test represents S100P analysis with the cut-off value of 99.8 ng/ml. Negative index test is less than the cut-off value and a positive index test is equal to or more than the cut-off value. *Endoscopic ultrasound-guided fine needle aspiration. †The FNA samples for the new device were obtained by 1 pass or by flushing out the residual material with air. A total amount of 1 or 2 passes by EUS-FNA were used for cytological diagnosis. ‡Bicinchoninic acid. §S100P protein analysis could not be quantified, due to low total protein concentrations (<10 mg/ml) which consisted of pancreatic adenocarcinoma in 3 patients, autoimmune pancreatitis in 1 and chronic pancreatitis in 1. ?Pancreatic adenocarcinoma.

Fig 4: S100P is down-regulated in the mid-secretory phase endometrium of participants from GnRH-ant group. A S100P gene expression in different groups from microarray data. B Immunohistochemical detection of S100P in the endometrium from the different groups, magnified in 200 times and the scale bar represents 50um. a. Negative control, b. Natural cycle control, c. GnRH antagonist group, d. GnRH agonist group; quantified in (C). D Location of S100P in endometrium by co-localization with vimentin and keratin. Red: S100P; Green: vimentin (b) and cytokeratin (f); Blue: nucleus. The scale bar represents 50um. E Western blot results of S100P and HOXA10 protein expression in endometrium from the natural cycle, GnRH-ant and GnRH-a groups. F Quantification of S100P protein expression. G Quantification of HOXA10 protein expression. *P < .05, **P<.01, ***P<.001

Fig 5: Knockdown of S100P induces apoptosis in endometrial epithelial cells. A Overexpression and knockdown of S100P mRNA was verified by Realtime PCR. B Overexpression and knockdown of S100P protein was verified and expression of Bcl-2 and Bax protein was analyzed by western blot. C Apoptosis rate was evaluated by flow cytometry after overexpression and knockdown of S100P in Ishikawa cells; results are quantified and analyzed in (D). NC: negative control using scramble sequence; KD: S100P knockdown; Mock: mock control transfected with empty plasmid vector; OE: S100P overexpression; *P < .05