Fig 1: Effects of high glucose, H2O2 and apocynin on the expression of key factors involved in mitogen-activated protein kinase signaling pathways. (A) Representative western blotting bands of Erk1, phospho-Erk1, Jnk1, phospho-Jnk1, p38 and phospho-p38. (B) Quantitative expression levels of Erk1 protein. (C) Quantitative expression levels of phospho-Erk1 protein. (D) Quantitative expression levels of Jnk1 protein. (E) Quantitative expression levels of phospho-Jnk1 protein. (F) Quantitative expression levels of p38 protein. (G) Quantitative expression levels of phospho-p38 protein. #P<0.05 vs. NC group; *P<0.05, as indicated. Erk1, extracellular signal-regulated kinase 1; phospho/phos-, phosphorylated; Jnk1, c-Jun N-terminal kinase 1; NC, normal control; Apo, apocynin; Glu group, cells were treated with 25 mM glucose; Glu+Apo group, cells were treated with 25 mM glucose and 100 µg/ml apocynin; H2O2 group, cells were treated with 100 nmol/l H2O2; H2O2+Apo group, cells were treated with 100 µg/ml apocynin and 100 nmol/l H2O2; Glu+H2O2 group, cells were treated with 25 mM glucose and 100 nmol/l H2O2; Glu+H2O2+Apo group, cells were treated with 25 mM glucose, 100 µg/ml apocynin and 100 nmol/l H2O2; a.u., arbitrary units.
Fig 2: Representative immunohistochemical images of GRB2 (A and B), GAB1 (D and E) and p-ERK1 (G and H) expression in HCC and adjacent non-neoplastic liver tissues (Original magnification×400).Statistical analyses of IRS for GRB2 (C), GAB1 (F) and p-ERK1 (I) immunostainings in HCC and adjacent non-neoplastic liver tissues. GRB2 positive staining was localized in the cell nucleus and cytoplasm, while GAB1 positive staining was localized in the cytoplasm of tumor cells in HCC tissues. Compared with the adjacent nonneoplastic tissues, the expression levels of GRB2 (IRS for HCC vs. nonneoplastic liver: 6.32±1.50 vs. 2.67±0.32, P<0.001) and GAB1 (IRS for HCC vs. nonneoplastic liver: 5.72±0.95 vs. 1.75±0.48, P<0.001) proteins were all significantly increased in HCC tissues. More interestingly, in all four groups according to the combined expression of GRB2 and GAB1, GRB2-high/GAB1-high patients expressed the highest level of p-ERK1 protein (all P=0.01).
Supplier Page from Abcam for Anti-ERK1 (phospho Y204) antibody