Fig 1: Protein kinase SRPK1, SRPK2, and CLK1 protein expression in normoxic and hypoxic HCT116 cells. Western blot showing SRPK1 (A), SRPK2 (B), and CLK1 (C) protein expression levels in normoxic and hypoxic cells. Bar plot represents the quantification of endogenous protein expression of 3 independent experiments when protein expression in cells grown under normal oxygen conditions is taken as 1. Error bars represent the amount of spread between the extremes of the data, ** p < 0.05. N—cells cultivated under normal conditions; H—cells cultivated under hypoxic conditions for indicated time (2 h, 4 h, and 24 h).