Fig 2: Silencing of IQGAP1 significantly attenuated MCDHF-induced liver injury and fibrosis(A) Liver sections were subjected to Sirius Red staining. Scale bars: 50 µm. (B) The area of fibrotic liver was quantified. (C and D) mRNA levels of Col1a1 (C) and Col1a3 (D) were examined. (E and F) Serum levels of AST (E) and ALT (F) were measured in MCDHF-fed mice. (G) A schema diagram of IQGAP1-mediated, S1P-induced BMSC migration. Data are presented as the means ± SEM. *p < 0.05 versus control group and #p < 0.05 versus MCDHF group with SCR siRNA (n = 5, per group).

Fig 3: IQGAP1 is involved in S1P-induced migration of BMSCs(A and B) The expression of IQGAP1 in BMSCs was detected by immunofluorescence (A) or western blot (B). HeLa cells was used as the positive control (B). Scale bars: 25 µm. (C) IQGAP1 was knocked down by small interfering RNA (siRNA) (40 nmol/L); the knockdown efficiency of IQGAP1 was studied by qRT-PCR (left) or western blot (right). (D) The migration ability of BMSCs was detected by Transwell chambers. (E) The representative images of BMSC migration. Scale bars: 100 µm. Arrow indicates migrating cell. (F) The representative images of microfilaments staining in BMSCs. Scale bars: 25 µm. BMSCs were stimulated with 10 nmol/L and 1 µmol/L S1P; the remodeling of microfilaments was examined by high-content analysis. (G and H) The fiber number (G) and fiber area (H) were analyzed by high-content analysis. Data are presented as the means ± SEM. All results were confirmed in three independent experiments at least. *p < 0.05 versus control group and #p < 0.05 versus S1P group (n = 3, per group).

Fig 4: IQGAP1-siRNA in hibits invasion of U251 and U373 cells. (A and B) The invasive ability of U251 cells was markedly suppressed by IQGAP1-siRNA. (C and D) The invasive ability of U373 cells was markedly suppressed by IQGAP1-siRNA. n=3. Data are presented as the mean ± standard deviation. Magnification, ×200. **P<0.01, ***P<0.001 vs. the control group; ##P<0.01 vs. the NC group. IQGAP1, IQ motif containing GTPase activating protein 1; siRNA, small interfering RNA; NC, negative control.

Fig 5: High expression of IQGAP1 in glioma tissues and cell lines. (A) mRNA expression levels of IQGAP1 in glioma tissues were significantly higher compared with in normal tissues (n=3). Data are presented as the mean ± standard deviation. ***P<0.001 vs. normal tissues. (B and C) IQGAP1 was more highly expressed in U251 and U373 cell lines compared with the others (n=3). Data are presented as the mean ± standard deviation. **P<0.05, ***P<0.001 vs. T98G and U87. IQGAP1, IQ motif containing GTPase activating protein 1.