Fig 1: Immunohistochemistry analysis of BMAL1, HIF-1a, ANG1, ANG2, and VEGF expression in glioma and normal tissues with different pathological grades.
Fig 2: Immunohistochemical analysis (IHC) on granulation tissue of rats showing the changes of Ang1, Src, and VE-cadherin expression. IHC (×40 magnification, scale bar = 50 µm) was performed on rats showing Ang1, Src, and VE-cadherin expression with treatment after anal fistulas for 3, 7, and 14 days.
Fig 3: Western Blot analysis of Ang-1 protein expression in the MI border regions of canines. (a) Myocardial Ang-1 and GAPDH protein levels quantified using a Western Blot 3 days after UTMD-mediated Ang-1 transfection. Lanes 1–5 represented groups A, B, C, D, and E, respectively. (b) Quantification of the Ang-1 protein relative expression levels was performed in the MI border regions of canines in all groups. The Ang-1 was normalized by GAPDH. The Ang-1 protein expression was the highest in group C; *P < 0.05 compared to the other four groups.
Fig 4: mRNA and protein expression analysis. (a) Quantitative representation of expression of Src, VE-cadherin, Ang1, and Tie-2 mRNA were evaluated. (b) Western blotting showing the expressions of Src, VE-cadherin, Ang1, and Tie-2 protein and corresponding semiquantitative analysis of protein level of Src, VE-cadherin, Ang1, and Tie-2. Data are expressed as mean ± SEM (n = 3) and analyzed by one-way ANOVA, P < 0.05. Data are expressed as mean ± SEM (n = 3) and analyzed by one-way ANOVA.
Fig 5: Real-time PCR detects Ang-1 mRNA expression in the MI regions of canines 3 days after transfection. The Ang-1 mRNA was normalized by GAPDH. The Ang-1 mRNA was higher in group C compared to the other groups, *P < 0.05 compared to the other groups.
Supplier Page from Abcam for Anti-Angiopoietin 1 antibody