Fig 1: CDCA content and inflammasome expression were both suppressed in MRP2-/- mice. (A) High and low concentrations of BAs in liver tissues of MRP2−/− mice (n = 5) and WT mice (n = 4) were detected by LC-MS (**p < 0.01 and ***p < 0.001). (B) The relative ratio of GCDCA and TCDCA to CDCA concentrations in MRP2−/− mice (n = 5) and WT mice (n = 4) determined by LC-MS. (p < 0.05 compared with WT). (C) Western blot analysis of inflammasome Pro-caspase-1 cleavage in liver tissues of MRP2−/− mice (n = 5) and WT mice (n = 4); β-actin was used as a loading control, and the band intensities were quantified by Image J software and shown as a bar graph. Quantification of the image was shown as means ± SD, and **p < 0.01 compared with WT. (D) Heat map analysis of the mRNA of 20 genes that control BA homeostasis in MRP2−/− mice (n = 3) and WT mice (n = 3) detected by qPCR. (E) The protein expression of FXRα, Cyp8b1, Cyp27a1, Sult2a1, Bsep, Mrp3, Ntcp, and BAAT in liver tissues of WT (n = 4) versus MRP2−/− mice (n = 5). GAPDH was used as a loading control, and the band intensities were quantified by ImageJ software and shown as a bar graph. Quantification of the image was shown as means ± SD, *p < 0.05, and ***p < 0.001 compared with WT. Abbreviations: BAs, bile acids; CDCA, chenodeoxycholic acid; FXR, farnesoid X receptor; TCDCA, taurochenodeoxycholic acid; WT, wild type.