Fig 1: Overexpression of SERPINA3 in human macrophages rescues overwhelming cytokines in response to LPS.a, b Real-time PCR analyses demonstrating cytokine transcripts in PBS- or LPS-treated monocyte-derived macrophages (a) or THP-1 cells (b) with or without SERPINA3- or SOCS1-lentivirus infection as indicated. Ctrl, Control. Data are shown as mean ± SD. Two-way two-sided ANOVA was performed for statistical analyses.
Fig 2: The roles of SERPINA3 in patients.a Box-and-whisker plot showing the LPS concentrations in the serums from healthy individuals and sepsis patients, n = 15 independent samples for each group. b Quantification analysis of m6A levels in the blood monocytes of healthy individuals and sepsis patients displayed by box-and-whisker plot, n = 15 independent samples for each group. c Box-and-whisker plot exhibiting SERPINA3 and cytokine mRNAs expression in the blood monocytes of healthy individuals and sepsis patients, n = 15 independent samples for each group. d–h Pearson’s correlation analysis among m6A levels, SERPINA3, TNF, IL-6, IL-1ß and IFN? in the peripheral blood monocytes from sepsis patients. i, j Pearson’s correlation analysis between SERPINA3 expression in the peripheral blood monocytes and clinical parameters of sepsis patients. k, l Pearson’s correlation analysis between m6A levels in the peripheral blood monocytes and clinical parameters of sepsis patients. Data are shown as mean ± SD. For box-and-whisker plots, the bottom line shows the lower quartile, top line the upper quartile, and whiskers the maxima/minima. Unpaired two-tailed Student’s t tests (a–c) and Pearson’s correlation (d–l) were used for statistical analysis.
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