Anti-DDX4 / MVH antibody [mAbcam27591] ab27591 from Abcam

Product Specs
ItemAnti-DDX4 / MVH antibody [mAbcam27591]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab27591
HostMouse, Nude mouse
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
ClonalityMonoclonal
ClonemAbcam27591
Antibody TypePrimary
Conjugate/TagUnconjugated
IsotypeIgG1
Quantity100 UG
Concentration1 mg/ml
Research AreaEpigenetics
Gene NameDDX4
ReactivityCommon marmoset, Human, Mouse
TargetDDX4 / MVH

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Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Zika virus infection leads to hormone deficiencies of the hypothalamic-pituitary-gonadal axis and diminished fertility in miceJournal of VirologyOctober 31, 2023Li-Bo Liu, Wei Yang, Jia-Tong Chang, Dong-Ying Fan, Yan-Hua Wu, Pei-Gang Wang, Jing Anmouse anti-ZIKV NS1 monoclonal antibody (1:500, GT5212, Invitrogen), mouse anti-DDX4 antibody (1:400, ab27591, Abcam), rabbit anti-SOX9 antibody (1:400, AB5535, Millipore), and rabbit anti-Oxytocin antibody (1:400,

(2) SRSF2 is required for mRNA splicing during spermatogenesisBMC BiologyOctober 23, 2023Wen-Long Lei, Zongchang Du, Tie-Gang Meng, Ruibao Su, Yuan-Yuan Li, Wenbo Liu, Si-Min Sun, Meng-Yu Liu, Yi Hou, Chun-Hui Zhang, et al.SYCP3 (mouse, sc-74569; Santa Cruz); γH2AX (rabbit, 9718; Cell Signaling Technology, Inc.); MVH (mouse, ab27591; Abcam); SOX9 antibody (rabbit, AB5535, Sigma-Aldrich); PLZF antibody (goat, AF2944, R&D Systems); SFRS2 polyclonal antibody

(3) The non-redundant functions of PIWI family proteins in gametogenesis in golden hamstersNature CommunicationsAugust 29, 2023Xiaolong Lv, Wen Xiao, Yana Lai, Zhaozhen Zhang, Hongdao Zhang, Chen Qiu, Li Hou, Qin Chen, Duanduan Wang, Yun Gao, et al.(1:200, lab produced); anti-PIWIL4 polyclonal antibodies (1:200, lab produced); anti-DDX4 (1:200, Abcam, ab27591); anti-TDRKH (1:80, Thermo Scientific, PA5-48098); anti-DCP1A (1:200, Santa Cruz, sc-100706), anti-DDX6

(4) PARP1-catalyzed PARylation of YY1 mediates endoplasmic reticulum stress in granulosa cells to determine primordial follicle activationCell Death DisAugust 15, 2023Wei Chen, Qiukai E, Bo Sun, Pengxue Zhang, Nan Li, Shujia Fei, Yingnan Wang, Shuting Liu, Xiaoqiu Liu, Xuesen Zhangbinding was performed overnight at 4 °C, including anti-DDX4 (1:200, #Ab13840, Abcam), anti-DDX4 (1:200, #Ab27591, Abcam), anti-GRP78 (1:200, #11587-1-AP, Proteintech), PARP1 (1:400, #66520-1-lg, Proteintech), anti-FOXL2

(5) Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cellsCell Reports MethodsAugust 1, 2023Kenyu Iwatsuki, Mami Oikawa, Hisato Kobayashi, Christopher A. Penfold, Makoto Sanbo, Takuya Yamamoto, Shinichi Hochi, Kazuki Kurimoto, Masumi Hirabayashi, Toshihiro KobayashiAB_2750924 GFP,Abcam,Cat# ab13970; RRID: AB_300798 dsRed,Takara Bio,Cat# 632496; RRID: AB_10013483 DDX4/MVH,Abcam,Cat# ab27591; RRID: AB_11139638 FOXA2/HNF3β,Cell Signaling,Cat# 8186S; RRID: N/A PNA,Vector Laboratories,Cat# RL-1072;

(6) In vitro oogenesis from murine premeiotic germ cells using a new three-dimensional culture systemCell Death DiscoveryJuly 31, 2023Lu Wang, Zi-Hui Yan, Tao-Ran He, Hai-Xia Liu, Yu-Kang Li, Yi-Lin Niu, Jun-Jie Wang, Massimo De Felici, Wei Ge, Wei Shenthe slides. After blocking for 30 min, the slides were incubated with the anti-DDX4 antibody (Abcam ab27591, UK) at 4°C overnight. We then washed the slides three times with TBST. After washing, the slides were

(7) The dynamic expression of SOX17 in germ cells from human female foetus and adult ovaries after specificationFront Endocrinol (Lausanne)July 28, 2023Ying-Yi Luo, Hui-Ying Jie, Ke-Jun Huang, Bing Cai, Xiu Zhou, Ming-Yi Liang, Can-Quan Zhou, Qing-Yun Mai12–14 hours. Primary antibody dilutions were as follows: SOX17 (1:20; #AF1924, R&D, USA), VASA (1:100; #AB27591, Abcam, USA), PHH3 (1:800; #AB47297, Abcam), and SCP3 (1:2000; #AB150292, Abcam). On Day 2, sections

(8) Deciphering the Molecular Characteristics of Human Idiopathic Nonobstructive Azoospermia from the Perspective of Germ CellsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)June 1, 2023Chen Y, Liu X, Zhang L, Zhu F, Yan L, Tang W, Zhang Z, Liu Q, Jiang H, Qiao J.PBST at room temperature for 1 h followed by incubation with primary antibodies against DDX4 (1:500, ab27591, Abcam), KI67 (1:500, ab21700, Abcam), LELP1 (1:100, ab229947, Abcam), TEX38 (1:100, 24429‐1‐AP, Proteintech),

(9) Single-cell RNA sequencing reveals the fragility of male spermatogenic cells to Zika virus-induced complement activationNature CommunicationsApril 29, 2023Wei Yang, Li-Bo Liu, Feng-Liang Liu, Yan-Hua Wu, Zi-Da Zhen, Dong-Ying Fan, Zi-Yang Sheng, Zheng-Ran Song, Jia-Tong Chang, Yong-Tang Zheng, et al.rabbit anti-mouse Caspase-1 antibody (1:500, Abcam, ab74279), mouse anti-mouse DDX4 (1:100, Abcam, ab27591), rabbit anti-mouse DDX4 antibody (1:100, Abcam, ab13840), rabbit anti-mouse S100A4 antibody (1:500,

(10) Chito-oligosaccharides and macrophages have synergistic effects on improving ovarian stem cells function by regulating inflammatory factorsJournal of ovarian researchApril 14, 2023Zheng K, Hong W, Ye H, Zhou Z, Ling S, Li Y, Dai Y, Zhong Z, Yang Z, Zheng Y.was performed according to the manufacturer's specifications. The primary antibodies used were MVH (ab27591, Abcam, USA), OCT4 (ab18976, Abcam, USA), TNF-α (EPR22598-212, Proteintech, USA), IL-2 (26,156–1-AP,

(11) TP63 gain-of-function mutations cause premature ovarian insufficiency by inducing oocyte apoptosisJournal of Clinical InvestigationMarch 1, 2023Chengzi Huang, Simin Zhao, Yajuan Yang, Ting Guo, Hanni Ke, Xin Mi, Yingying Qin, Zi-Jiang Chen, Shidou Zhaoincubation at 4°C overnight with the following primary antibodies: anti-DDX4 (1:600 dilution, Abcam, catalog ab27591); anti-p63 (1:200 dilution, Abcam, catalog ab124762); anti–cleaved-PARP1 (1:400 dilution, Cell Signaling Technology

(12) Multi-Omics Approaches for Revealing the Epigenetic Regulation of Histone H3.1 during Spermatogonial Stem Cell Differentiation In VitroInternational journal of molecular sciencesFebruary 7, 2023Liu L, Li H, Wang M, Zhang X, Ren J, Yuan Y, Sha J, Guo X.PLZF (1:200, ab305064, Abcam), GFP (1:200 Millipore, Merck, Darmstadt, Germany), DDX4 (1:500 Abcam, ab27591, Minneapolis, MI, USA), and STRA8 (1:200 Abcam, ab49602, Minneapolis, MI, USA). The samples were then

(13) Mice 3D testicular organoid system as a novel tool to study Zika virus pathogenesisVirologica SinicaFebruary 1, 2023Wei Yang, Chen Zhang, Yan-Hua Wu, Li-Bo Liu, Zi-Da Zhen, Dong-Ying Fan, Zheng-Ran Song, Jia-Tong Chang, Pei-Gang Wang, Jing Ananti-mouse cleaved caspase-3 (1:500, Cell Signaling Technology, 9664S), mouse anti-DDX4 (1:100, Abcam, ab27591), rabbit anti-mouse DDX4 (1:100, Abcam, ab13840), mouse anti-mouse Vimentin (1:100, Abcam, ab8978),

(14) Immunity and reproduction protective effects of Chitosan Oligosaccharides in Cyclophosphamide/Busulfan-induced premature ovarian failure model miceFrontiers in immunologyJanuary 1, 2023Li X, Ye H, Su T, Hu C, Huang Y, Fu X, Zhong Z, Du X, Zheng Y.including anti-GAPDH (1:1000, ab181602, Abcam), anti-fragilis (1:200, ab18976, Abcam), anti-MVH (1:5000, ab27591, Abcam), anti-TNF-α (1:200, ab1793, Abcam), anti-IL-2 (1:200, ab11510, Abcam), anti-IL-4 (1:1000, 66142-1-Ig,

(15) SRSF1-mediated alternative splicing is required for spermatogenesisInternational journal of biological sciencesJanuary 1, 2023Lei WL, Li YY, Du Z, Su R, Meng TG, Ning Y, Hou G, Schatten H, Wang ZB, Han Z, et al.SYCP3 (mouse, sc-74569; Santa Cruz); γH2AX (rabbit, 9718; Cell Signaling Technology, Inc.); MVH (mouse, ab27591; Abcam); SOX9 antibody (rabbit, AB5535, Sigma-Aldrich); PLZF antibody (goat, AF2944, R&D Systems); SFRS1

(16) Targeting APLN/APJ restores blood-testis barrier and improves spermatogenesis in murine and human diabetic modelsNature communicationsNovember 28, 2022Song K, Yang X, An G, Xia X, Zhao J, Xu X, Wan C, Liu T, Zheng Y, Ren S, et al.(Proteintech, 66009-1-Ig, 1:400), mouse-anti-VIMENTIN (Proteintech, 60330-1-Ig, 1:50), mouse anti-DDX4 (Abcam, ab27591, 1:500), rabbit anti-SOX9 (Millipore, AB5535, 1:200), rabbit anti-SYCP3 (Abcam, ab15093, 1:400), mouse

(17) Activation of embryonic/germ cell-like axis links poor outcomes of gliomasCancer cell internationalNovember 26, 2022Ma Z, Zhang F, Xiong J, Zhang H, Lin HK, Liu C.(MAB2018R-100, R&D), anti-Nanog (NB100-58842, Novus Biologicals), anti-Nanos3 (ab70001, Abcam), anti-DDX4 (ab27591, Abcam), SCP3 (ab 15,093, Abcam) or anti-DAZL (NB100-2437, Novus biologicals). Next, the cells were

(18) Effects and potential mechanism of Ca(2+)/calmodulin‑dependent protein kinase II pathway inhibitor KN93 on the development of ovarian follicleInternational journal of molecular medicineOctober 1, 2022Yu J, Xie X, Ma Y, Yang Y, Wang C, Xia G, Ding X, Liu X.follows: Anti-CaMKII antibody (1:200; cat. no. ab52476; Abcam), DEAD-box helicase 4 (DDX4; 1:50; cat. no. ab27591; Abcam) and anti-forkhead box L2 (FOXL2) antibody (1:100; cat. no. NB100-1277; Novus Biologicals, LLC).

(19) Identification of curcumin as a novel potential drug for promoting the development of small ovarian follicles for infertility treatmentPNAS NexusJuly 8, 2022Yu Zhao, Yihui Zhang, Dongteng Liu, Haiwei Feng, Xiaohui Wang, Jiajun Su, Yuanqing Yao, Ernest H Y Ng, William S B Yeung, Raymond H W Li, et al.(#G3G4; Developmental Studies Hybridoma Bank [DSHB], Iowa City, IA, USA) at 1:200 dilution and anti-MVH (ab27591; Abcam, Cambridge, UK) at 1:200 dilution. Granulosa cell isolation and culture Ovaries at PD13 were

(20) Z-DNA is remodelled by ZBTB43 in prospermatogonia to safeguard the germline genome and epigenomeNature Cell BiologyJuly 4, 2022Yingying Meng et al.mouse anti-Z22 (AbsoluteAntibody, cat. no. Ab00783-3.0) 1:10,000; mouse anti-DDX4 (Abcam, cat. no. ab27591) 1:100; rabbit anti-PGC7 (Abcam, cat. no. ab19878) 1:200; mouse anti-OCT3/4 (Santa Cruz Biotechnology,

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Anti-DDX4 / MVH antibody [mAbcam27591] from Abcam

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Anti-DDX4 / MVH antibody [mAbcam27591] from Abcam

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