Fig 1: The NRAP NM_198060.3:c.4504C > T:p.Arg1502* variant in the proband. Pedigree of proband family (A) and IGV view of the NRAP NM_198060.3:c.4504C > T:p.Arg1502* variant found by whole exome sequencing (B) and chromatogram from direct sequencing by the Sanger method (C). Pedigree: squares represent males and circles represent females. An arrowhead denotes the proband. Solid black symbols denote dilated cardiomyopathy and open symbols unaffected individuals. The presence or absence of the NRAP variant is indicated by a +/- symbol.
Fig 2: Western blot of NRAP protein from heart samples of proband and control. RVW - right ventricular wall, S - septum, LVW - left ventricular wall. Control sample was taken from heart transplantation recipient without truncating variants in NRAP gene.
Fig 3: Expression of regulatory proteins in response to AAV treatment. a A representative Western blot depicting NRAP expression, KLHL41 expression, and GAPDH expression in vehicle-treated (LEDL, LTC) and AAV-treated (REDL, RTC) CTRL and Neb cKO mice. b Changes in KLHL41 expression following AAV treatment. Both CTRL and Neb cKO TC muscles experience an increase in protein expression. Similarly, CTRL EDL muscles experience an increase, but Neb cKO EDL muscles remain unchanged. c Changes in NRAP expression following AAV treatment. While CTRL TC muscles experience a trending increase toward higher expression, control EDL muscles have a significant increase in protein expression. Neb cKO EDL muscles experience a significant decrease in NRAP protein. (n = 5, 6 mice)
Supplier Page from Abcam for Anti-NRAP antibody