Fig 1: Hepcidin protein levels are decreased in the livers of diabetes prone animals. Expression levels of the two predicted hepatokines (A) Hamp and (D) Shbg in the livers of DP and DR mice were measured by qRT-PCR (n = 16 per group). (B, E) Western blot of hepatic hepcidin and sex hormone binding (SHBG) protein levels and (C, F) quantification was performed by densitometric analysis relative to a-tubulin and GAPDH, (n = 5 per group). Differences between the groups were calculated by Student's t-test with Welch's correction *p < 0.05, ***p < 0.001, ****p < 0.0001.