Fig 1: SNN eliminates promotion of miR-491-3p on Rb cells proliferation, migration and invasion. a MTS assay of cell proliferation of Y79 and Weri-Rb1 cells, or transfected with miR-491-3p mimics, miR-491-3p + vector or miR-491-3p + oe-SNN. b Colony formation assay of cell proliferation in Y79 and Weri-Rb1 cells, or transfected with miR-491-3p mimics, miR-491-3p + vector or miR-491-3p + oe-SNN. Left images are clone formation on the culture dish of Y79 and Weri-Rb1 cells, or transfected with miR-491-3p mimics, miR-491-3p + vector or miR-491-3p + oe-SNN; right chart is the number of clones quantitatively analyzed. All results are from three independent experiments. c Left images are staining of cell migration of Y79 and Weri-Rb1 cells, or transfected with miR-491-3p mimics, miR-491-3p + vector or miR-491-3p + oe-SNN under inverted microscope (magnification, × 100); right chart is statistical analysis of these clone cultures. d Left images are staining of cell invasion of Y79 and Weri-Rb1 cells, or transfected with miR-491-3p mimics, miR-491-3p + vector or miR-491-3p + oe-SNN under inverted microscope (magnification, × 100); right chart is statistical analysis of these clone cultures. All results are from three independent experiments. Bars represent mean ± SD (n = 3, *p < 0.05)
Fig 2: SNN eliminates influence of miR-491-3p on apoptosis and EMT of Rb cells. a Left images are the FACS assay of apoptosis in Y79 and Weri-Rb1 cells, or transfected with miR-491-3p mimics, miR-491-3p + vector or miR-491-3p + oe-SNN. Right chart is the quantitative analysis of the apoptosis rate of these images. b E-cadherin, vimentin and N-cadherin protein levels in Y79 and Weri-Rb1 cells, or transfected with miR-491-3p mimics, miR-491-3p + vector or miR-491-3p + oe-SNN. c In situ immunocytochemistry of Rb cell line transfected with miR-491-3p mimics, miR-491-3p + vector or miR-491-3p + oe-SNN (magnification, × 400). Left image showed the morphology changes of cells, right image is statistical analysis of left image. All results are from three independent experiments. Bars represent mean ± SD (n = 3, *p < 0.05)
Fig 3: Stannin is targeted by miR-491-3p and upregulated in vivo and in vitro. a The prediction result was taken from the intersection of the 4 miRNA target prediction databases. b Prediction of pairing location of miRNA miR-491-3p to mRNA SNN. c Luciferase reporter assay of Y79 or Weri-Rb1 cells with SNN-WT or SNN-MUT transfected with mimic NC or miR-491-3p mimics. All results are from three independent experiments. d Western blotting of the expression of SNN in Y79 and Weri-Rb1 cells transfected with mimics NC, miR-491-3p mimics. e RT-qPCR analysis of expression of SNN in Rb tissues and adjacent noncancerous tissues. All results are from three independent experiments. f RT-qPCR analysis of expression of SNN in ARPE-19, Weri-Rb1 or Y79 cells. All results are from three independent experiments. g RT-qPCR analysis of transfection rate of oe-SNN in the Y79 or Weri-Rb1 cells. All results are from three independent experiments. Bars represent mean ± SD (n = 3, *p < 0.05)
Supplier Page from Abcam for Anti-Stannin antibody