Fig 1: NUDCD1 knockdown inhibited the EMT process in PC cells. (A–D) NUDCD1 knockdown decreased the expression of N-cadherin and vimentin and upregulated the expression of E-cadherin in PANC-1 cells. (E–H) NUDCD1 knockdown decreased the expression of N-cadherin and vimentin, and upregulated the expression of E-cadherin in Patu8988 cells. ***p<0.001, ****p<0.0001.
Fig 2: Expression and roles of NUDCD1 in PC. (A) NUDCD1 mRNA expression levels were significantly higher in PDAC tissues (n = 179) than in normal pancreatic tissues (n = 171). (B, C) NUDCD1 upregulation was significantly correlated with shorter overall survival and shorter disease-free survival (p = 0.041 and 0.037, respectively). (D) NUDCD1 was overexpressed in PDAC tissues relative to adjacent tissues as detected by IHC. (E) NUDCD1 upregulation was observed in different PC cell lines by qRT-PCR. (F, G) High NUDCD1 expression was observed in different PC cell lines by Western blot. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
Fig 3: NUDCD1 knockdown suppressed the invasion and migratory abilities of PC cells. (A–D) NUDCD1 knockdown inhibited the migration of Patu8988 and PANC-1 cells in the wound healing assay. (E–G) NUDCD1 knockdown inhibited the invasion of PANC-1 and Patu8988 cells in the transwell assay. **p<0.01, ***p<0.001.
Fig 4: NUDCD1 knockdown inhibited the growth of PC in vivo. (A–C) NUDCD1 knockdown remarkably reduced tumor volume and weight. (D–G) NUDCD1 knockdown decreased the expression of N-cadherin and vimentin and increased the expression of E-cadherin in tumor tissue. **p<0.01, ***p<0.001, ****p<0.0001.
Fig 5: NUDCD1 knockdown inhibited the proliferation of PC cells. (A, B) The interference effects of three siRNAs on NUDCD1 were confirmed by qRT-PCR. (C–G) NUDCD1 knockdown inhibited the proliferation of PANC-1 and Patu8988 cells. (H–K) NUDCD1 knockdown inhibited the colony formation of Patu8988 and PANC-1 cells. (L–N) Western blot analysis showed that NUDCD1 knockdown decreased the expression of Ki-67 in PANC-1 and Patu8988 cells.**p<0.01, ***p<0.001, ****p<0.0001.
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