Fig 1: Identification of signaling molecules downstream of Adenosine-A2AR pathway in striatum/NAc. (A) Model of A2AR signaling pathway in D2R-MSN; (B) Scheme for the phosphoproteomic analysis used to identify candidate substrates downstream of the A2AR pathway. Striatal/accumbal slices were stimulated with CGS21680 (5 µM) for 5 min after pretreatment with quinpirole (1 µM) for 10 min to induce A2AR-mediated phosphorylation of the phosphoprotein and then subjected to 14-3-3? affinity chromatography followed by LC-MS/MS; (C) Stimulation of adenosine A2AR promotes PKA-mediated phosphorylation of Rap1gap, and Rasgrp2 in striatal/accumbal slices; (D) Motif analysis of the identified phosphopeptides downstream of A2AR signaling is shown.
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