Fig 1: Overall survival rate (OS) of the patients according to the studied biomarkers. A and B: OS rate of LUSC patients and association with CLCA2, SPATS2 expression. C and D: OS rate of LUAD patients and association with ST6GALNAC1 and Adipophilin expression
Fig 2: Progression free survival rate (PFS) of the patients according to the studied biomarkers. A and B: PFS rate of LUSC patients and association with CLCA2, SPATS2 expression. C and D: PFS rate of LUAD patients and association with ST6GALNAC1 and Adipophilin expression
Fig 3: SPATS2 is associated with key proteins associated with esophageal squamous cell carcinoma progression. (A) Representative western blots of cyclin E, MMP-9, N-cadherin and P53 in Eca109 cells were (B) quantified after knockdown of SPATS2 using si-SPATS2#2. **P<0.01 vs. si-con. con, control; MMP, matrix metalloproteinase; si, small interfering; SPATS2 spermatogenesis-associated serine rich 2.
Fig 4: Immunohistochemical profile of lung squamous cell carcinoma (LUSC) according to the results of the studied markers. A: Diffuse cytoplasmic expression of CLCA2 in LUSC X400. B: Diffuse cytoplasmic expression of SPATS2 in LUSC X400. C: Negative expression of ST6GALNAC1 in LUSCX400. D: Negative expression of Adipophilin in LUSCX400
Fig 5: Silencing SPATS2 represses Eca109 cell proliferation, colony formation, migration and invasion capabilities. (A) mRNA levels and (B) representative western blots indicated that SPATS2 expression decreased after cells were transfected with si-SPATS2, compared with control. (C) Western blotting densitometry. (D) Cell proliferation was reduced after transfection with si-SPATS2 compared with control. (E) Representative images of colonies formed. (F) Number of colonies formed was significantly decreast after transfection with si-SPATS2 compared with si-con. **P<0.01 vs. si-con. con, control; si, small interfering; SPATS2 spermatogenesis-associated serine rich 2. (G) Representative images of migratory and invasive cells. (H) Statistical analysis indicated that migratory and invasive cell numbers declined after knockdown of SPATS2, compared with si-con. Scale bar=200 µm, **P<0.01 vs. si-con. con, control; si, small interfering; SPATS2 spermatogenesis-associated serine rich 2.
Supplier Page from Abcam for Anti-SPATS2 antibody