Fig 1: Gene Expression Analysis of Inflammatory Mediators and Transcription Factors after c-Myc knockdown. A. Time-dependent changes in the expression of pro-inflammatory genes Angptl4, Cxcl12, Mdk, Tgfb2, Tnfsf15 and Vcam1 in endothelial cells three (black bars) and six (gray bars) days after c-Myc knockdown. B. Transcription factor gene expression profiling 6 days after c-Myc knockdown. Results are expressed as fold-change relative to NS-Control. Gene expression data were normalized to at least two endogenous control genes. C. Network pathway analysis of inflammatory mediators and transcription factors induced by c-Myc knockdown. (n = 4–5) (*p<0.05, **p<0.005).
Fig 2: Protein Expression Analysis of Pro-Inflammatory Markers. A. Representative western blot image showing time-dependent changes in the expression of Tnfsf15, Vcam1, and Stat1 after c-Myc knockdown. Expression of Stat1 was analyzed 6 days after knockdown. Actin was used as loading control (n = 4). B. Expression analysis of Angptl4, Tgfb2 and Cxcl12 in cell lysates and concentrated supernatants (Spnt) by ELISA 6 days after knockdown (n = 4). NS, control; KD, knockdown. *p<0.05, **p<0.005.
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