Fig 1: (A) Western blot assay of B4GalT1 expression in tumor and peritumoral tissue.(B) Quantification, and (C) Quantitative RT-PCR assay for mRNA level of B4GalT1 in different tissues. (D) Western blot assay of B4GalT1 expression in SVG-P12, A172, U251 and U87 cells. (E) Quantification, and (F) Quantitative RT-PCR assay for mRNA level of B4GalT1 in different cells. Protein and mRNA levels were normalized to GAPDH. (Tumor or U87 cells vs. para-tumor or SVG-P12 cells, *p < 0.05, n = 6 per group, all data was represented as Mean ± Standard error).
Fig 2: (A) Tumor volume in 28 days period and (B) Tumor weight at 28 day of Con, sh-B4GalT1 and control-shB4GalT1 mice. (C) Western blot assay of IDH1, MGMT and ki-67 expression in vivo. (D) Quantification of IDH1, MGMT and ki-67 expression. (E) Survival analysis of Con, sh-B4GalT1 and control-shB4GalT1 mice. Protein levels were normalized to GAPDH. (sh-B4GalT1 vs. other group, *p < 0.05, n = 6 per group).
Fig 3: (A) The heat map of GBM patients of Merged Cohort of LGG and GBM (TCGA, Cell 2016) in TCGA system. (B) Expression profile assay in GBM and normal tissue. (C) Overall survival analyses of high level and low level B4GalT1 in GBM patients. (D) Disease free survival analyses of high level and low level B4GalT1 in GBM patients. (E) Pair-wise gene correlation analysis of B4GalT1 and Beclin-1. (F) Pair-wise gene correlation analysis of B4GalT1 and LC3.
Fig 4: The potential mechanisms of apoptosis and autophagy by B4GalT1 mediated in glioblastoma. B4GalT1 probably could reduce the related proteins of apoptosis and autophagy expression just like Bax, Bcl-2, caspase-3, Beclin-1 or LC3 s, weaken the apoptosis and autophagy progress to tumor cells, and cut down lives of mice.
Fig 5: The expression levels of specific marker genes of diverse luminal clusters examined by scRNA-seq analysis and immunostaining in PCa tissue. a Violin plots displaying the expression levels of each luminal representative markers in each cluster. b Expression levels of representative markers for each luminal cluster plotted onto the UMAP. Color key from gray to red indicates relative expression levels from low to high. c Immunostaining showing the cytological localization of each luminal cluster cells in representative PCa tissues. Blue fluorescence represents nucleus stained with DAPI; green fluorescence represents type 1 luminal cells stained with anti-SLC45A3; red fluorescence represents type 2 luminal cells stained with anti-CP; purple fluorescence represents type 3 luminal cells stained with anti-B4GALT1
Supplier Page from Abcam for Anti-B4GALT1 antibody