Fig 1: Endothelial Nf2 deletion produces more tip-like ECs, while it reduces stalk-like ECs in tumor vessels.(A) Diagram depicting tamoxifen administration and implantation of LLC cells into WT and Nf2i?EC mice, harvesting tumors and sorting of tumor ECs at day 21, and performing single-cell RNA analysis. (B) Uniform manifold approximation and projection plots visualizing four clusters of tumor ECs by unsupervised clustering in WT and Nf2i?EC tumor EC datasets. (C) Pie chart depicting compositions of tumor EC subpopulations in WT and Nf2i?EC datasets. (D) Significantly enriched pathways and GO terms in Nf2i?EC over WT tumor ECs. Bars indicate significance defined as -log10adjusted P values. (E) Heatmap visualizing scaled expression of genes related to tip cells, hypoxia, internalization, and ERK signaling in WT and Nf2i?EC tumor ECs. (F and G) Representative images and comparisons of PlGF+/CD31+ tip-like tumor ECs and ACKR1+/CD31+ stalk-like tumor ECs between WT and Nf2i?EC mice. Scale bars, 100 µm. Each dot indicates a value from one mouse and n = 6 mice per group from two independent experiments. Vertical bars indicate means ± SD. P values by two-tailed t test.