Fig 1: DAND5 promoted tumor proliferation and angiogenesis in vitro and in vivoA. Representative pictures of pseudocapillary formation in matrigel from HUVECs in 0.1% FBS exposed to breast cancer cell culture at 12 h after cell seeding. B. Quantification of pseudocapillaries obtained by counting numbers of complete circles/wells. Numbers represent the mean of 6 samples ± SEM of three experiments run in triplicate. C. MDA-MB231 DAND5 and knock down stable cell lysates and T47D DAND5 stable cell lysates were immunoblotted using DAND5 antibody. D. MDA-MB231 wild type, DAND5 and knock down stable cells were seeded in 96 well plate and cultured for the times as indicated, the cell growth was analyzed with CCK-8 assay. *P <0.05. **P<0.001. E. Inhibition of DAND5 by shRNA inhibited the vascularization of tumors in mice. The images were reconstructed using the filtered back projection (FBP) algorithm. Tumorigenesis was obtained after injection of MDA-MB231 cells or MDA-MB231 shDAND5 cells. F. Image of representative tumors from MDA-MB231 or MDA-MB231 shDAND5 xenografts harvested at end point. G. Images to visualize positive staining of DAND5 in xenograft tumor harvested at end point. Bars: 20 um, magnification × 400.