Fig 1: Alteration of the morphology and redistribution of cytoskeleton of human NSCLC and osteosarcoma cells by biologic miR-124-3p. (A) Phase contrast images (10× magnification) of cells taken with a digital camera under an Olympus CK×31 inverted microscope at different time points following various treatments. A549 and 143B cells gradually changed from spindle to oval shapes or largely compressed by miR-124-3p, with the decrease of cell numbers and clusters. (B) Confocal imaging studies revealed a redistribution of cytoskeletal networks by miR-124-3p, as manifested by F-actin (red) and PLEC (green) staining. In addition, the microvilli or lamellipodia- and filopodia-like structures (white arrowheads) within the 143B cells were sharply impaired by miR-124-3p. Nuclei stained with DAPI are labeled in blue. Scale bar, 10 µm.