Fig 1: Gene expression changes in Rev-erba-/- liver and comparison with GR target genes. (A) Venn-diagram based on the RNA sequencing analysis of Rev-erba-/- and control (wild-type, WT) livers at ZT8 (red) and ZT20 (blue). (B) Comparison of the 846 genes altered at ZT8 (red) in Rev-Erba-/- liver with datasets from Phuc Le et al. (2005) (yellow and green, ZT4–ZT8). The yellow circle represents gene expression alterations in the liver after dexamethasone treatment assessed by microarray. The green circle represents genes detected by a ChIP-on-chip assay using an antiserum raised against GR to reveal chromatin bound by GR. (C) Verification of three of the eight genes in common to all three evaluations: alcohol dehydrogenase 1 (Adh1), glycine N-methyltransferase (Gnmt), and hepatic lipase C (Lipc). Values are mean±s.e.m., n=3. *P<0.05, **P<0.01 (two-way ANOVA and Tukey's post-test). (D) Verification of the bona fide REV-ERBa target gene Insig2a and the GR target gene I?Ba (Nfkbia) (values are mean±s.e.m., n=3. *P<0.05, **P<0.01 (two-way ANOVA and Tukey's post-test). (E) Chromatin immunoprecipitation assay revealing the time-dependent interaction of GR with the promoters of Per1, Insig2, Adh1, Gnmt and Lipc. Values are mean±s.e.m., n=8, *P<0.05 (one-way ANOVA with Bonferroni-post test).