Fig 2: Effect of WG on PTH1R. (A) Western blot analysis of PTH1R. (B) Quantitative analysis of PTH1R relative to ß-actin. SW1353 cells were pre-treated with indicated concentration of WG or DXM for 4 h in 0.2% BSA media followed by IL-1ß for 24 h. (C) Western blot analysis of cytosolic and nuclear fractions using HDAC4 antibody, (D) quantitative analysis of cytosolic HDAC4 relative ß-actin, (E) quantitative analysis of nuclear HDAC4 relative to Lamin A/C. SW1353 cells were pre-treated with WG or DXM for 4 h in 0.2% BSA media in the presence or absence of PTH (7–34) followed by IL-1ß for 24 h. (# p < 0.05 and ## p < 0.01 vs. control; ** p < 0.01 vs. IL-1ß; † p < 0.05 vs. WG).

Fig 3: Analysis of gene expression in the cartilage of Cbfßf/f Col2a1-Cre mice by IF. (A-C) Immunofluorescence (IF) staining of the growth plates from tibial frozen sections of P7 Cbfßf/f Cbfßf/fCol2a1-Cre (ff/?) and WT (ff) mice with anti-CyclinD1 (A), anti-Ihh (B), and anti-PPR (C). (A-C) High-magnification figures were co-presented on the right panels. (D) Western blot analysis of the expression of Cbfß, PPR, Ihh, PCNA, Cyclin D1, Runx2, and Runx3 in the limb epiphyseal cartilages of newborn Cbfßf/fCol2a1-Cre and WT mice. ß-Tubulin was used as a loading control. (E) Quantification of protein expression level normalized to ß-Tubulin in “D”. P, proliferative zone; R, resting zone; H, hypertrophic zone; pre-H, pre-hypertrophic zone. Data in A-C are representative for 3 mice per group.