Fig 2: Location of each Th subset in the spleens of NOD mice and immunoblots of the indicated molecules. a Immunohistochemical staining for T-bet (Th1 cells), GATA3 (Th2 cells), Foxp3 (Treg cells), and RORγ (Th17 cells). The panels on the upper side show the higher magnifications (bar = 100 μm.) of the spleens. b The detection of NFAT, NF-κB, Foxp3, p-Smad2/3, Smad2/3, p-ERK, and ERK protein expression levels was conducted using western blot analysis. β-actin was used as the internal control

Fig 3: Effect of cDC manipulation on the balance of the Th1/Th2 response. The mRNA and protein expression levels of T-bet and GATA-3 were measured by reverse transcription-quantitative PCR and western blotting to evaluate the balance of the Th1/Th2 response. (A) mRNA expression of T-bet and (B) GATA-3. ß-actin was used as a reference gene. (C) Representative blots from western blotting analysis of T-bet and GATA-3 protein expression. (D) Protein expression levels of T-bet and (E) GATA-3. ß-actin was used as a reference protein. Data are presented as the mean ± standard deviation (n=3). *P<0.05 vs. Control; #P<0.05 vs. ARDS; &P<0.05 vs. FLT3L+ARDS; and $P<0.05 vs. lestaurtinib+ARDS. cDC, classical dendritic cell; Th, T helper cell; T-bet, T-box-expressed-in-T-cells; GATA-3, GATA binding protein 3; ARDS, acute respiratory distress syndrome; FLT3L, Fms-like tyrosine kinase 3-ligand.

Fig 4: Localization of each Th subset in the spleens of the MRL/lpr mice. Immunohistochemical staining for T-bet (Th1 cells), GATA3 (Th2 cells), Foxp3 (Treg cells), and RORγ (Th17 cells). Panels on the left ose on the right show the higher-magnification images (bars = 50 μm) of the areas surrounded by dotted lines.

Fig 5: Correlation analysis of VSIG4 expression and immune infiltration in PDAC. A. GSEA analysis of VSIG4-related pathways according to TCGA. B. Representative IHC images of VSIG4, CD8A, FOXP3, T-bet and GATA3 expression in Renji TMA. C. Patients were divided into two groups according to the expression of VSIG4, and the column bar graphs showed the correlation between CD8A, FOXP3, T-bet, GATA3, and VSIG4. Bar graphs are presented as mean + SD (unpaired two-tailed Student t test; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001)