Fig 1: E006AA cells are tumorigenic and immunogenic in nude mice, and do not require a physiologic level of testosterone for growth.(A) Inoculation of E006AA cells into male nude mice compared to male NOG-SCID mice. (B) Transfer of tumor tissue derived from an established E006AA xenograft tumor from a male NOG-SCID mouse to intact male Nude and NOG-SCID mice. (C) The growth of E006AA and CWR22 human prostate cancer xenograft tumors in castrated vs. intact male mice at eight weeks. (D) Histological analysis (H&E) and immunostaining of E006AA xenograft tissue sections for AR, PSA, p63, and CK5 (images taken at 20× and 40×). (E) Western blot analysis of CK8 and CK18 expression in LNCaP, PC-3, E006AA and S006AA cells. ß-Actin served as a loading control.