Fig 1: Presence of inflammation and immune cells at the injury sites in the tunica media under prolonged hyperlipidemia. (A) Corresponding aorta tissue sections positive for lipid clusters and VV (described in Figure 3A) were stained with markers of immune cells and inflammation, CD45, CD163, and CD68 (red). TA, tunica adventitia; TM, tunica media; TI, tunica intima; L, lumen. Scale bar: 100 µm. (B) Arterial tissues at indicated sites show the detection of immune cell markers, including CD45, CD4 and CD8 T cells, CD19 (B cells), Ly6G (granulocytes/neutrophils) and NK1.1 (NK cells). Scale bar: 20 µm. (C) Corresponding injected sites under hyperlipidemic condition were stained for detection of chemokines (MCP1, CCL21) and metalloproteinase markers (MMP2, MMP9 and, ADAM10). Nuclei DAPI (blue). Scale bar: 100 µm.