Fig 2: p38-MAPK pathway-related protein and mRNA expression, and immunohistochemical expression of p-CHOP10 on day 7 post-small bowel transplantation. Protein and mRNA expression of (A) MLK3 to p-MLK3, (B) MKK3 to p-MKK3, (C) p38 to p-p38, (D) ATF2 to p-ATF2, (E) CHOP10 to p-CHOP10, (F) MEF2C to p-MEF2C, (G) PCNA and (H) ZO-1 in each group. (I) Expression of related protein of the p38-MAPK pathway as demonstrated by western blotting. *P<0.05.. (J) Immunohistochemical expression of p-CHOP10 on day 7 post-small bowel transplantation: (a) NBST, (b) IsoT, (c) NS, (d) MSCs, (e) Ad-HO/MSCs and (f) Ad-(CXCR3 + HO)/MSCs groups. Expression was significantly lower in the Normal group than in the other groups, with the exception of the Ad-(CXCR3 + HO)/MSCs group (P<0.05); expression in the IsoT group was lower than that in the NS, MSCs and Ad-HO/MSCs groups (P<0.05). Expression in the Ad-HO/MSCs group was lower than that in the MSCs and NS groups (P<0.05). Expression in the NS group was the highest, and expression in the Ad-(CXCR3 + HO)/MSCs group was significantly lower than that in all experimental groups. MSCs, bone marrow mesenchymal stem cells; Ad, adenovirus; CXCR3, CXC-chemokine receptor CXCR3; HO-1, heme oxygenase-1; L, lymphocytes; S, S203580; IsoT, isogeneic transplantation of the small bowel from genetically identical host; NS, injected intravenously with normal saline (0.9% sodium chloride solution) CHOP10, CHOP10, C/EBP homologous protein-10; MLK3, mixed lineage kinases 3; MKK3, mitogen-activated protein kinase kinase 3; ATF2, activating transcription factor 2; MEF2C, myocyte enhancer factor type 2C; PCNA, proliferating cell nuclear antigen; p-, phosphorylated.