Fig 1: Analysis of osteoclastogenic markers in the bone marrow cells after proton and 56Fe exposure. A) Representative immunofluorescence images (magnification 400x) depicting RANK (osteoclast marker), RANKL (osteoclastogenesis marker) and OPG (osteoblast marker) expressing cells. B) Quantification of RANKL/OPG ratio from immuno-stained sections. C) Quantification of osteoclast/osteoblast ratio from immuno-stained sections. Each bar represents mean ± SEM values from 10 to 12 HPF with n = 5 mice/group. ∗ denotes p < 0.05 compared to the control group and ∗∗ denotes p < 0.05 between irradiated groups.