Fig 1: Immunoblotting results and the metabolic network of differential metabolites between PIGinH11 (TG) and wild-type (WT) pigs after 2-months of HFHSD. (A,B) Protein expression of total PNPLA3 (porcine PNPLA3 and humanized PNPLA3I148M) was detected using liver samples. The degree of Akt activation was investigated by detecting phosphor-Akt. The lower ratio of phosphorylated Akt Ser473 to total Akt in TG samples indicated suppressed Akt signaling. The protein expressions of GPAT3, MOGAT2, and TMEM16F were detected using liver (C,D) and skeletal muscles (E,F) from TG and WT pigs. The differential metabolites’ network was shown in (G). GPAT3, Glycerol-3-Phosphate Acyltransferase 3, also refers as MAG1. MOGAT2, Monoacylglycerol O-Acyltransferase 2. TMEM16F, anoctamin 6, also refers as ANO6. Data were shown as mean ± SD, n = 4 per group, * p < 0.05, ** p < 0.01.