Fig 2: Iba1+ pixel-cluster density increased in the EG eye laminar and retrolaminar regions. Representative control (left) and EG (right) eye single IHC section images (A, B) and animal-specific mean intensity heat maps (C) for Iba+ pixel-cluster density for NHP 2. A Control and EG eye fluorescent full section images (see Fig. 3A for the clinical location of these sections), with magnified views of the white outlined regions shown in B. C MBP intensity heat maps for NHP2 based on mean data for four section images from each eye reveal diffuse qualitative decreases in both retrolaminar RL1 and RL2 subregions (see Fig. 3) which achieve significance by statistical analysis (see Table 2, Supplementary Table S1).

Fig 3: Graphic display of experiment-wide qIHC by target and region. CNPase, MBP, and GFAP fluorescence intensity per unit area as well as NucBlue and Iba1 pixel-cluster per mm2 are displayed. Values in the boxes are 25th to 75th percentile and whiskers are plotted minimum to maximum. Significance is shown by asterisks (also see Table 2). In NHPs 1 and 4, the retrolaminar nerve within the paraffin sections was short and the RL2 region therefore could not be analyzed for all antibodies except MBP (shown by underlying black triangle). Experiment-wide data for the RL2 region for CNPase, GFAP, NucBlue pixel-cluster density, and Iba1 cluster density is thus based on two animals (NHPs 2 and 3). See Supplementary Table S1 for actual data.