Fig 1: Lineage tracing of adult iris cell types with Sox10-Cre and Wnt1-Cre.(A) Bar graphs showing the fraction of cells that express nuclear-localized H2B-GFP, indicating excision of the LoxP-stop-LoxP cassette from the Cre reporter R26-LSL-tdT-2A-H2BGFP. Iris cell types were identified by immunostaining for the nuclear-localized markers listed. The intensities of GFP and the cell type markers vary among nuclei. A nucleus was considered to exhibit co-localization if both signals were present, regardless of intensity. For sphincter muscle, the number of cells scored for each Cre driver ranged from 120 to 140. For each of the other cell types, the number of cells scored for each Cre driver ranged from 960 to 2300. (B) Iris flat mounts (left) and cross sections (right) from Sox10-Cre;R26-LSL-tdT-2A-H2BGFP mice showing mutually exclusive localization of GFP and OTX1 +2 (‘OTX’) (upper panels) and partial co-localization of GFP and TFAP2B (lower panels). (C) Iris flat mounts (left) and cross sections (right) from Wnt1-Cre;R26-LSL-tdT-2A-H2BGFP mice showing partial co-localization of GFP and TFAP2B (upper panels) and minimal co-localization of GFP and NeuN (lower panels). All images are at the same magnification. Scale bars, 100 µm.