VLDLR Antibody (6A6) - BSA Free NBP1-78162 from Novus Biologicals, a Bio-Techne Brand

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Scientific reports
April 13 2021

Fig 1: Effect of very low-density lipoprotein receptor (Vldlr) knockout and dietary protein restriction on VLDLR in mice. Typical western blotting images of VLDLR and loading control (Actb and Tubulin) in the liver, heart, gastrocnemius muscle, and epididymal white adipose tissue (eWAT) (A,B,C,D, respectively), and the total amount of type 1 and type 2 VLDLR (E,F,G,H) obtained by digitizing the band density of western blotting in wild-type (WT) or Vldlr-deficient (KO) mice fed the control (20C) or protein-restricted (3C) diet. Photographs of two samples are shown, and a black line in the lower image of muscle VLDLR and tubulin delineate the boundary between the gels processed in parallel. The brightness and contrast of the images depicting liver VLDLR, eWAT VLDLR and muscle tubulin were adjusted equally across the entire image. Unmodified full-length photographs of the blots are shown in Figs. S1 and S6. Values are expressed as a relative value with the WT 20C group as 1 and the mean ± SEM, n = 10 per group. **P < 0.01, *P < 0.05 versus 20C.
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Scientific reports
April 13 2021

Fig 2: Effect of very low-density lipoprotein receptor (Vldlr) knockout and dietary protein restriction on Vldlr mRNA in mice. Relative Vldlr mRNA levels in (A) liver (total Vldlr), (B) heart, (C) gastrocnemius muscle, (D) epididymal white adipose tissue (eWAT), (E) liver (type 1 and 2) and (F) liver (type 1 Vldlr) in wild-type (WT) or Vldlr-deficient (VLDLR KO) mice fed a control (20C) or protein-restricted (3C) diet. M in (E) indicates a molecular marker. The unmodified full-length photograph of the gel in (E) is shown in Fig. S5. Values are expressed as a relative value with the WT 20C group as 1 and the mean ± SEM, n = 10 per group. **P < 0.01, *P < 0.05 versus 20C.
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Scientific reports
April 13 2021

Fig 3: Effect of very low-density lipoprotein receptor (Vldlr) knockout and dietary protein restriction on growth in mice. (A) Body weight, (B) food intake, (C) liver weight, (D) heart weight, (E) gastrocnemius muscle weight and (F) epididymal white adipose tissue (eWAT) weight in wild type (WT) or Vldlr-deficient (VLDLR KO) mice fed control (20C) or protein-restricted (3C) diet. Values are the mean ± SEM, n = 10 per group. Results of two-way analysis of variance to evaluate the significant effects of Vldlr deficiency (Vldlr), protein restriction (PR), and the interaction of Vldlr × PR are shown in the tables; **P < 0.01, NS: not significant (A,B). Results of the difference between the two groups are shown. **P < 0.01, *P < 0.05 versus 20C (C–F).
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Scientific reports
April 13 2021

Fig 4: Effect of very low-density lipoprotein receptor (Vldlr) knockout and dietary protein restriction on Asns mRNA in mice. Relative Asns mRNA levels in (A) liver, (B) heart, (C) gastrocnemius muscle, and (D) epididymal white adipose tissue (eWAT) in wild-type (WT) or Vldlr-deficient (VLDLR KO) mice fed the control (20C) or protein-restricted (3C) diet. Values are expressed as a relative value with the WT 20C group as 1 and the mean ± SEM, n = 10 per group. **P < 0.01, *P < 0.05 versus 20C.
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Scientific reports
April 13 2021

Fig 5: Effect of very low-density lipoprotein receptor (Vldlr) knockout and dietary protein restriction on liver and plasma lipids in mice. Liver and plasma triglyceride (TG) (A,D), cholesterol (CHO) (B,E), and phospholipid (PL) (C,F) in wild-type (WT) or Vldlr-deficient (VLDLR KO) mice fed the control (20C) or protein-restricted (3C) diet. Values are expressed as the mean ± SEM, n = 10 per group. **P < 0.01, *P < 0.05 versus 20C.