Fig 1: Expression of meiotic and germline proteins in MS and giant cells found by Immunofluorescence—representative images for at least three experiments: (A) a tetraploid cell nucleus enriched with MOS-kinase (sc-86) colocalized and juxtaposed with CYCLIN B1 (DOX-D2); (B) an attachment of MOS to the centrosomes and microtubules of the tripolar mitosis (DOX-D4); (C) MOS and α-TUBULIN form a monopolar spindle in the early prophase (DOX-D4); (D) MOS is attached to interphase centrosomes (arrow) and shows a remnant of a monopolar spindle in MS (asterisk) [12]; (E) the restituting nucleus in MS becomes poor with MOS and CYCLIN B1 (DOX-D4); (F–H) a giant cell in MS releasing cytoplasmic DNA shows the enrichment of the cell nucleus with DMC1 (meiotic recombinase) and REC8 (meiotic cohesin) (DOX-D19); (I) REC8 grains are scarcely inserted in the kinetochore chains in the MS cell (arrow) (DOX-D4); (J) DMC1 grains are scarcely inserted in the MS cell (DOX-D7; [12]); (K) a giant cell enriched with OCT4A in the cell nucleus (a monoclonal Ab) and OCT4B in the cytoplasm (DOX-D5); and (L) a giant cell enriched with the germ markers, DDX4/VASA in the cell nucleus and FRAGILIS in the cytoplasm (DOX-D7). Bars = 10 µm.