Fig 1: Increased mEPSC amplitude and levels of excitatory postsynaptic receptors in model rats. (A) Diagram showing model generation and patch clamp/western blot analysis. (B) Representative mEPSC (left) and mIPSC (right) traces recorded in the ACC. (C) Statistical analysis indicating significantly increased amplitude but not frequency of mEPSCs in model rat ACC (frequency: Control, 1.92 ± 0.06 Hz, PP, 1.71 ± 0.124 Hz, p = 0.136; amplitude: Control, 12.26 ± 0.48 pA, PP, 14.22 ± 0.55 pA, p = 0.012; Control, n = 21 cells of five rats; PP, n = 18 cells of five rats). (D) Identical mIPSC frequency and amplitude in control and model ACC (frequency: Control, 2.00 ± 0.11 Hz, PP, 1.95 ± 0.11 Hz, p = 0.20; amplitude: Control,13.06 ± 0.59 pA, PP, 11.67 ± 0.55 pA, p = 0.09; Control, n = 22 cells of five rats; PP, n = 22 cells of five rats). (E) Example of immunoblots of ACC extracts probed with anti-GluR1, GluR2, GluR4, NR1, and NR2B antibodies and quantification of the immunoblots revealing significant increases in levels of GluR1 (p = 0.014) and NR1 (p = 0.024), but not GluR2, GluR4 and NR2B. (F) Representative mEPSC (left) and mIPSC (right) traces in the HIPP. (G) Statistical analysis showing significantly increased mEPSC amplitude, but not frequency, in model rats (frequency: Control, 1.36 ± 0.12 Hz, p = 0.23; PP, 1.31 ± 0.15 Hz, p = 0.23; amplitude: Control, 10.89 ± 0.78 pA, PP, 12.67 ± 0.84 pA, p = 0.02; Control, n = 21 cells of five rats; PP, n = 20 cells of five rats). (H) Identical mIPSC frequency and amplitude in HIPP of control and model rats (frequency: Control, 0.98 ± 0.14, PP, 0.76 ± 0.18, p = 0.43; amplitude: Control, 10.71 ± 0.53, PP, 9.82 ± 0.59, p = 0.36; Control, n = 22 cells of 5 rats; PP, n = 22 cells of five rats). (I) Example of immunoblots probed with anti-GluR1, GluR2, GluR4, NR1, and NR2B antibodies and quantification of the immunoblots revealing a significant increase in levels of NR1 (p = 0.029). Values represent mean ± SEM. One-way ANOVA was used for mEPSCs and mIPSCs, and two-sample t-test for western blotting. *p < 0.05, ***p < 0.001.