Fig 1: TRAPPC12 and TRAPPC8 were TRAPPIII-specific subunits.(A) HeLa cell lysates were subjected to immunoprecipitation using antibody specific to TRAPPC9 and TRAPPC12 and rabbit-IgG as negative control. The different subunits of TRAPP complexes in the immunoprecipitates were detected by immunoblotting using the indicated antibodies specific to these proteins. (B) A comparison of subunit composition between TRAPPC6A IP, and TRAPPC9 and TRAPPC12 IP’s. Immunoprecipitations with mouse monoclonal TRAPPC6A antibody was performed and mouse IgG was used as negative control. IP’s with TRAPPC9 and TRAPPC12 were similar to those shown in (A). In both panels, approximately 1% of the input lysates and 20% of the immunoprecipitates were loaded onto the SDS-PAGE.