Fig 2: Representative images show that treatment with NCA results in an increased expression of γ-H2AX in CHEK2-null HCT116 cells as compared to HCT116 cells. DMSO was used as a negative control.

Fig 3: Representative images show that treatment with NCA results in an increased expression of cleaved caspase-3 in CHEK2-null HCT116 cells as compared to HCT116 cells. DMSO was used as a negative control.

Fig 4: (A) Images of DAPI-stained nuclei of CHEK2-null HCT116 cells after treatment with DMSO and NCA. (Objective: 4×). Scale bar: 1000 μm. (B) Bar graph shows treatment with NCA leads to significantly (p < 0.001) reduced cell numbers of CHEK2-null HCT116 cells as compared to the DMSO control.

Fig 5: (A) Images of DAPI-stained nuclei of CHEK2-null HCT116 cells after treatment with siRNA for GAPDH (negative control) and PRDX2. (Objective: 4×). Scale bar: 1000 µm. (B) Bar graph shows that treatment with siPRDX2 leads to significantly (p < 0.001) reduced cell numbers of CHEK2-null HCT116 cells as compared to HCT116 cells, while there was no significant difference in cell numbers between CHEK2-null HCT116 cells and HCT116 cells after treatment with siGAPDH. GAPDH was the negative control.