Western blot was performed using Anti-CNR2 Polyclonal Antibody (Product # PA5-18428) and a 40kDa band corresponding to CNR2 was observed across tissues tested except in Mouse Brain which is reported to be low expressing tissue for CNR2. Tissue extracts (30 µg lysate) of Mouse Spleen (Lane 1), Mouse Thymus (Lane 2), Mouse Brain (Lane 3), Mouse Ovary (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG Heavy Chain Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Western blot was performed using Anti-CNR2 Polyclonal Antibody (Product # PA5-18428) and a 40kDa band corresponding to CNR2 was observed across tissues tested except in Mouse Brain which is reported to be low expressing tissue for CNR2. Tissue extracts (30 µg lysate) of Mouse Spleen (Lane 1), Mouse Thymus (Lane 2), Mouse Brain (Lane 3), Mouse Ovary (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supplier Page from Thermo Fisher Scientific for CNR2 Antibody