Fig 1: LXA4 may interfere with autophagy and inhibit inflammatory hyperactivation via activation of the NF-?B signalling pathway.(a) Western blot assay was used to detect the expression of phospho-p52, phospho-p65 and I?B in macrophages stimulated by PgLPS (LPS) and pre-treated with different concentrations of LXA4 (100 and 150nM), rapamycin or 3-MA. Data were compared to beta-actin.(b) By comparison with control (con), semi-quantitative analysis of the phospho-p65 level in macrophages stimulated by LPS and pre-treated with different concentrations of LXA4 (100 and 150nM), rapamycin or 3-MA (n = 3) Values were normalised to beta-actin.(c) By comparison with control (con), semi-quantitative analysis of I?B in in macrophages stimulated by LPS and pre-treated with different concentrations of LXA4 (100 and 150nM), rapamycin or 3-MA (n = 3). Values were normalised to beta-actin.*P < 0.01 vs control; **P < 0.05 vs control; # P < 0.05 vs Pg LPS.