Fig 2: YAP directly regulates myelin and myelin-related protein expression(a) Western blots of differentiated primary rat Schwann cells following infection with GFP control or constitutively active YAP S127A Flag adenovirus. GFP and Flag panels confirm infection in four independent cultures of each condition. Myelin proteins EGR2 (∼50 kDa) expression is increased, MPZ (∼27 kDa) is downregulated and MBP (∼45 kDa) is unchanged by YAP activation. A positive control for MBP from whole sciatic nerve extract is shown in insert, right. MAG (∼100 kDa) is also upregulated as is expression of proteins with relevance to myelination, Rab11a (∼20 kDa) and Laminin1 (∼175 kDa), in response to YAP activation. Actin (∼48 kDa) was used to verify loading. (b) Quantification and statistical analysis of the change in selected protein expression in primary RSCs following differentiation and infection with dominant active YAP S127A, relative to levels in cells infected with GFP control (normalized to 1, dotted line). (c) HEK 293T cells were transfected with plasmids expressing Mpz or Egr2 promoter driving Firefly luciferase expression, and either GFP, YAP alone or YAP and TEAD isoforms together. Luciferase levels were normalized to cells transfected with control GFP (three independent experiments in triplicates). Statistical analysis compares each transfection with the GFP infection for each promoter tested. a.u., arbitrary units. Statistical two-tailed Student's t-tests: *P<0.05; **P<0.01; ***P<0.001; NS, P>0.05 not significant (NS). Error bars show s.e.m.

Fig 3: Intra-nerve injections of AAV2/9-sh1 and -sh2 in CMT1A rats normalize MPZ expression back to WT rat level.a Representative images of Western blot showing MPZ protein levels (upper panel) and total protein as loading control (lower panel) in sciatic nerve lysates from WT ctr.sh, CMT1A ctr.sh, CMT1A sh1 or CMT1A sh2 three months after injection. b Graph shows mean MPZ level in sciatic nerve lysates normalized to total protein level loaded as loading control (n = 7 animals per group). Statistical test shows one-way ANOVA followed by Tukey’s post hoc, two-sided. *p = 0.0122 between WT ctr.sh and CMT1A ctr.sh, **p = 0.0028 between CMT1A ctr.sh and CMT1A sh1, **p = 0.0022 between CMT1A ctr.sh and CMT1A sh2; ns, not significant; arb. units, arbitrary unit. All error bars show SEM. Source data are provided as a Source Data file.