Western blot was performed using Anti-Arp3 Polyclonal Antibody (Product # PA5-17288) and an ~47 kDa band was observed across the panel tested except Mouse and Rat Skeletal Muscle. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF-7 (Lane 2), PC-3 (Lane 3), U-87 MG (Lane 4), HeLa (Lane 5), HEL 92.1.7 (Lane 6), tissue extracts (30 µg lysate) of Mouse Thymus (Lane 7), Mouse Skeletal Muscle (Lane 8), Rat Thymus (Lane 9) and Rat Skeletal Muscle (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).