Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), A549 (Lane3), K-562 (Lane 4), Jurkat (Lane 5), MOLT4 (Lane 6) and HCT116 (Lane 7). The blot was probed with Mouse anti-MCM2 Monoclonal Antibody (Product # MA5-12333, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 125 kDa band corresponding to MCM2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).