Fig 1: Determination of pluripotency markers by immunofluorescence. Nuclear colocalization of OCT4 in WT (A–A”) and PSEN1 E280A (B–B?) iPSCs, in WT (C) and PSEN1 E280A MenSCs (D), and in WT (E) and PSEN1E280A WJ-MSCs (F). Colocalization of SOX2 in WT (G–G?) and PSEN 1 E280A iPSCs (H–H?), in WT (I) and PSEN 1 E280A MenSCs (J), and in WT (K) and PSEN 1E280A WJ-MSCs (L). Colocalization of Nanog in WT (M–M?) and PSEN 1 E280A iPSCs (N–N?), in WT (O) and PSEN 1 E280A MenSCs (P), and in WT (Q) and PSEN 1 E280A WJ-MSCs (R). Colocalization of Klf4 in WT (S–S?) and PSEN 1 E280A iPSCs (T–T?), in WT (U) and PSEN 1 E280A MenSCs (V), and in WT (W) and PSEN 1 E280A WJ-MSCs (X). Quantitative data showing the nuclear mean fluorescence intensity for OCT4 (Y), SOX2 (Z), NANOG (AA), and KLF4 (AB). The figures represent one out of three independent experiments. The data are expressed as the mean ± SD; significant values were determined by one-way ANOVA with Tukey’s post hoc test; ns: not significant. Image magnification, 20×. Representative immunocytochemistry images of mesoderm germ layer stained for Vimentin (AC–AH), ectoderm stained for ectoderm stained for Nestin (AI–AN), and endoderm stained for CXCR4 (AO–AT) in WT and PSEN 1 E280 A mutation from iPSCs, MenScs, and WJ-MSCs. Nuclei are stained with Hoechst (blue). Scale bars 25 µm.