Fig 1: Interaction of CRMP2 with brain mitochondria. (A–C) Immunocytochemistry staining with anti-CRMP2 antibody (A, red) and visualization of mitochondria with mitochondrially targeted enhanced yellow fluorescent protein (mito-eYFP) (B, green) illustrates co-localization of CRMP2 and mitochondria (yellow, C) in cultured striatal neuron from FVB/NJ mouse. (D,E), CRMP2 binds to synaptic brain mitochondria isolated from FVB/NJ mice. Mitochondria were purified either on discontinuous 26/40% Percoll gradient (D) or on continuous 30% Percoll gradient (E) as described in the Materials and Methods. H: homogenate; C: cytosolic fraction; Mtc: mitochondrial fraction. MEK1/2, calnexin, ß-tubulin, Histone H3, and COX IV are cytosolic, ER, microtubule, nuclear, and mitochondrial markers, respectively. Representative western blots from 3 independent experiments are shown.