Fig 1: Multiomics analysis reveals quantifiable differences in protein and miRNA abundances in the Test group pan- and neuron-derived exosomes. (A) Differences in the abundances of proteins present in the salivary exosomes of Test group participants pre- and post-work shifts are illustrated via a volcano plot. The log10 (abundance ratio: post-work/pre-work) is plotted against -log10 (p value). (B) A Venn diagram shows the overlap of proteins found in exosomes isolated with pan-exosomal or neuron-selective exosomal markers for a single participant. (C) The protein fold change for the single Test group participant is shown when using global proteomics on exosomes isolated by a pan-exosome set of antibodies (Global MS (exosome abs: CD9, CD63, CD81); blue bars) or targeted MS on four corresponding to the four proteins (STVSSLLQK/BPIFA2, SQVVAGTNYFIK/CSTB, TVTINCPFK/PIGR, LDIDSPPITAR/PKM) present in exosomes isolated using an antibody to a neuron-specific exosome marker (Targeted MS (neuronal ab: CD171); orange bars). (D) Differences in the abundances of miRNAs present in the salivary exosomes of participants pre- and post-work shift are illustrated via a volcano plot. The log10 (abundance ratio: post-work/pre-work) is plotted against -log10 (p-value). (E) Fold change of miR-519d and miR-1296 as measured using the NanoString platform (y-axis) shows a moderate and strong positive correlation (miR-519d R2 = 0.50, r (5) = 0.70, p = 0.01881; miR-1296 R2 = 0.95, r (5) = 0.97, p = 0.0062) with fold change as measured by qPCR (x-axis).