Fig 1: Cellular diversity in the nonsensory ampulla epithelium.(a) Highlights the position of the nonsensory epithelial cells of the ampulla in UMAP space relative to the whole dataset. (b) Cluster analysis in nonsensory ampulla epithelium. (c) Expression of markers relative to cell cluster and developmental time. Additional color bars indicate whether markers are known or novel in specificity or implicated in vestibular diseases and dysfunction. (d) The relative contributions of cell clusters to the nonsensory ampulla epithelium at the indicated developmental ages. (e) Wnt3 RNA ISH localizes to a distinct population of cells in top of the roof of the P1 Swiss Webster ampulla. (f) Wnt3 IF in vibratome-sectioned crista localizes to the top of the roof of the P0 B6 ampulla and extends into the semicircular canal. (g) Wnt3 IF in a whole mount P7 B6 crista bisected to show the apical surface of the rooftop en face. (h) Phalloidin staining in P3 B6 ampulla whole mount. Note that roof cells in the Wnt3+ domain are smaller than surrounding epithelial cells. Brackets in f–g indicate the Wnt3+ rooftop domain. Scale bars = 50 µm.