MUC5AC Antibody MA5-12178 from Thermo Fisher Scientific

Published Figures
Supplier Images

Register or Sign In to View Figures.

Cell stem cell
September 05 2019

Fig 1: scRNA-Seq Analysis Defines the Molecular Signature and Lineage Relationships of Actively Cycling IsthSCs(A) t-SNE map showing the result of the consensus clustering (Kiselev et al., 2017) applied to scRNA-seq data from Stmn1+ cells. Based on marker gene expression, 9 clusters were classified into 5 states or lineages: G1/S phase; G2/M phase; pit cell lineage (PL); neck cell lineage (NL); and secretory cell lineage (SL).(B) t-SNE maps of Stmn1+ cells showing expression of representative genes for cell proliferation (Stmn1 and Mki67), cell cycle (Cdt1 and Ccnb1), and differentiation (Muc5ac and Muc6). The color bar indicates log2-transformed normalized read counts.(C) Pseudotime trajectory of Stmn1+ cells inferred by Slingshot (Street et al., 2018). This algorithm suggests that Stmn1high cells differentiate toward two sublineages, pit (Stmn1low Muc5achigh) and neck (Stmn1low Muc6high) cells.(D) Expression of marker genes along the pseudotime trajectory in (C). Proliferation marker (Mki67 and Stmn1) expression decreases along the trajectory from Stmn1high subpopulations toward Stmn1low Muc5achigh and Stmn1low Muc6high subpopulations. Muc5ac expression increases and Muc6 expression decreases along the path toward pit cell identity (top panel) and vice versa toward neck cell identity (bottom panel), reflecting bipotential differentiation of progeny derived from IsthSCs. Gene expression is represented as auto-scaled, log2-transformed normalized read counts.(E) Consensus matrix from SC3 clustering that justifies the identification of 6 subclusters (S1–S6) from the Stmn1high cluster (top left in B). Similarity 0 (blue) in the color bar means that the two cells are always assigned to distinct clusters, whereas similarity 1 (red) means that the two cells are always assigned to the same cluster.(F) UMAP map showing the 6 subclusters (S1–S6) of the Stmn1high cluster. Based on marker gene expression, S2 and S3 are primed toward the PL, whereas S6 are primed toward the NL.(G) Scatterplots of the correlation with the representative pit cell (pit cell correlation) and neck cell (neck cell correlation) for the 6 subclusters S1–S6. The p values (p) from the binomial test indicate the statistical significance of the bias of the correlations of a cluster between pit cell and neck cell lineages. N = 18 cells (S1), 82 cells (S2), 32 cells (S3), 56 cells (S4), 29 cells (S5), and 65 cells (S6) were used for the binomial test.(H) The subclusters S1–S6 in the pseudotime trajectory inferred by Slingshot where undifferentiated IsthSCs are primed toward early sublineage-restricted progenitors.(I) Gene expression trajectories along the pseudotime trajectory for two lineages. The lineages S4-S5-S1-S2-S3 and S4-S5-S1-S6 are pit cell and neck cell lineages, respectively. The represented expression values are log2-transformed normalized read counts followed by the z transform. The subcluster ID of cells is denoted by colors along the pseudotime orders in the bar on the x axis. Muc5ac and Muc6 show opposite behaviors in the two lineages. The expression of Cib1, Ndufb8, and Psma7 is highest at the start of pseudotime and decreases along the trajectory leading to early sublineage restriction.(J) Examples of undifferentiated IsthSCs and early sublineage-restricted progenitors in the isthmus region of the mouse stomach corpus glands based on mRNA expression detected by RNAscope. Red, Muc5ac; green, Muc6; grey, Ki67. “Ki67+Muc5achigh,” “Ki67+Muclow,” and “Ki67+Muc6high” indicate (1) Ki67+ cells expressing Muc5ac at high levels (PL progenitor); (2) Ki67+ cells expressing both Muc5ac and Muc6 at low levels, but not zero (IsthSC); and (3) Ki67+ cells expressing Muc6 at high levels (NL progenitor).See Figure S7 and Table S4.
Register or Sign In to View Figures.

Cells
November 18 2021

Fig 2: Activation of NOTCH3 signaling promotes club cell differentiation. (A–D) scRNA-seq analysis of HBECs cultured on ALI for 9 days. (A) UMAP clustering using K-means 6 clustering approach of 11,567 cells from a single HBEC donor. (B) Expression of KRT5 (basal cell marker). (C) Expression of SCGB1A1 (club cell marker). (D) Expression of NOTCH3. (E) Experimental design. HBECs (n = 6 normal, nonsmoker donors) were infected on air–liquid interface (ALI) with either control lentivirus (Lenti-Control) or lentivirus expressing the activated NOTCH3 NICD (Lenti-NICD3). The cells were then harvested as a function of time (ALI days 0, 3, 5 and 7) for subsequent analysis by qPCR and bulk RNA-seq. (F) GFP positivity of Lenti-Control and Lenti-NICD3 infected cells. Scale bar = 100 µm. (G) Heatmap showing temporal expression of markers for basal (KRT5), club (SCGB1A1), goblet (MUC5AC) and ciliated (FOXJ1) cells in response to NICD3 expression by bulk RNA-seq. Scale bar represents log2 fold change in expression in Lenti-NICD3 vs. Lenti-Control infected cells. (H) qPCR of SCGB1A1 (club cell marker) expression in Lenti-Control and Lenti-NICD3 infected cells from each HBEC donor. (I) Pathways enriched in the list of 692 NICD3-responsive genes identified by bulk RNA-seq on the basis of Ingenuity Pathway Analysis (IPA). Shown are the top 10 IPA-enriched pathways based on p-value (negative log10-transformed).
Register or Sign In to View Figures.

Nature communications
October 05 2022

Fig 3: Organoids and organoid-derived monolayers represent different regions of the gastric units.a UMAP visualisations of scRNA-seq of ex vivo isolated human gastric units (left, 1022 cells), gastric organoids (centre, 602 cells) and organoid-derived monolayers (right, 696 cells). EEC enteroendocrine cells. Cells are colour-coded according to the annotated clusters. b Dot plot showing the expression of cell type-specific marker genes within the clusters as identified in panel (a). c Expression of the pit cell marker MUC5AC and neck cell marker MUC6 colour-coded and projected on top of the UMAP visualisations as in panel a (data was normalized using ‘LogNormalized’ Seurat method for the expression of the gene within the dataset).
Register or Sign In to View Figures.

Disease models & mechanisms
November 01 2022

Fig 4: Identification of SARS-CoV-2-infected cells in the lungs of K18-hACE2 mice and Syrian golden hamsters. (A) Representative immunofluorescence images for N protein, AGER (type 1 alveolar cell marker), LAMP3 (type 2 alveolar cell marker) and 4',6-diamidino-2-phenylindole (DAPI; nuclear marker) in the lungs of K18-hACE2 mice 2 days after SARS-CoV-2 infection. Scale bars: 40 µm. (B) Percentage of infected cells (N protein+) per total positive cells in the alveolar lineage (AGER+ or LAMP3+) in SARS-CoV-2-infected K18-hACE2 mice at a 40× HPF (n=6 per group). All data are presented as the mean±s.e.m. P-values were obtained by two-tailed unpaired Student's t-test (***P<0.001). (C) Representative immunofluorescence images for N protein, SCGB1A1 (club cell marker), acetylated TUBA1A (Ac-TUBA1A; ciliated cell marker) and DAPI in the lungs of Syrian golden hamsters 2 days after SARS-CoV-2 infection. Scale bars: 40 µm. (D) Percentage of infected cells (N protein+) per total number of positive cells in the bronchus lineage (SCGB1A1+ or Ac-TUBA1A+ or MUC5AC+ or CHGA+) in SARS-CoV-2-infected Syrian golden hamsters in a 40× HPF (n=6 per group). All data are presented as the mean±s.e.m. P-values were obtained by two-tailed unpaired Student's t-test (***P<0.001, versus goblet cells; ###P<0.001, versus neuroendocrine cells). (E) Representative in situ hybridization images of human ACE2 (hACE2) in the lungs of non-infected K18-hACE2 mice and Syrian golden hamsters. Scale bars: 50 µm. (F) Co-staining images of in situ hybridization for S gene and immunohistochemical images of TUNEL in the lungs of K18-hACE2 mice and Syrian golden hamsters 2 days after SARS-CoV-2 infection. The black arrows indicate TUNEL+/S gene+ cells. Scale bars: 50 µm. (G) Percentage of TUNEL+/S gene+ cells relative to total S gene+ cells in SARS-CoV-2-infected K18-hACE2 mice and Syrian golden hamsters in a 40× HPF (n=6 per group). All data are presented as the mean±s.e.m. P-values were obtained by two-tailed unpaired Student's t-test (***P<0.001).
Register or Sign In to View Figures.

Annals of translational medicine
September 01 2022

Fig 5: Comparison of positive expressions of Muc5AC in gastric tissue of rats in each group (IHC, ×100, ×400 of the small box). JPYQF-L, Jian-Pi-Yi-Qi-Fang low-dose group; JPYQF-M, Jian-Pi-Yi-Qi-Fang middle-dose group; JPYQF-H, Jian-Pi-Yi-Qi-Fang high-dose group; IHC, immunohistochemistry.

Be the first to write a review!

Citations:

(300)

Figures:

Supplier Page

Supplier Page from
Thermo Fisher Scientific for
MUC5AC Antibody

The MUC5AC Antibody from Thermo Fisher Scientific is a Mouse Monoclonal antibody to MUC5AC. This antibody recognizes Homo Sapiens, Human, Mouse, Rat, Chicken/Bird, Feline, Non-Human Primate, Porcine, Rabbit, and Other Mammalian antigen. The MUC5AC Antibody has been validated for the following applications: Immunocytochemistry, Immunohistochemistry, and Immunohistochemistry - fixed.

Product Specs
ItemMUC5AC Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMA5-12178
ApplicationsImmunocytochemistry, Immunohistochemistry (Paraffin)
IsotypeIgG1 kappa
Brand/TrademarkABR (now Thermo Scientific Pierce Antibodies)
ReactivityChicken, Feline, Human, Mouse, Non-human primate, Porcine, Rabbit, Rat, Rodent
Quantity500 µL
Conjugate/TagUnconjugated
TargetMUC5AC
FormatPBS with 0.05% BSA and 0.05% sodium azide; pH 7.4
Clone45M1
HostMouse
ClonalityMonoclonal
NCBI Full Gene Namemucin 5, subtypes A and C, tracheobronchial/gastric, mucin 5AC, oligomeric mucus/gel-forming
Concentration0.2 mg/mL
ImmunogenM1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b-) patient
NCBI Gene AliasesleB, mucin, AABR07006032.1, MUC5, 2210005L13Rik, TBM, MGM
Storage Temperature4° C
PurityProtein A/G
Antibody TypePrimary
Storage4° C

Add to Compare List

Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host–pathogen interactions in the bat airway epitheliumEmerging Microbes & InfectionsDecember 31, 2023Louisa L.Y. Chan, Akshamal M. Gamage, Chee Wah Tan, Kai Sen Tan, Jing Liu, Douglas Jie Wen Wen Tay, Randy Jee Hiang Hiang Foo, Laurent Rénia, De Yun Wang, Lin-Fa Wangincluding mouse anti-acetylated α-tubulin (6-11B-1) (Cat#T7451, Sigma, USA), mouse anti-MUC5AC (45M1) (Cat#MA5-12178, ThermoFisher Scientific, USA) and rabbit anti-TP63 (EPR5701) (Cat#ab124762, Abcam, UK) diluted in staining

(2) Human-derived air-liquid interface cultures decipher Alzheimer's disease-SARS-CoV-2 crosstalk in the olfactory mucosaJ NeuroinflammationDecember 14, 2023Muhammad Ali Shahbaz, Suvi Kuivanen, Riikka Lampinen, Laura Mussalo, Tomáš Hron, Táňa Závodná, Ravi Ojha, Zdeněk Krejčík, Liudmila Saveleva, Numan Ahmad Tahir, et al.1:2000",Sigma-Aldrich,T6793-100UL; 108923 Mouse monoclonal MUC5AC antibody (45M1); Dilution 1:200,Thermo Fisher Scientific,MA5-12178; WD3205962 Mouse monoclonal human Cytokeratin 18 antibody (810811); Dilution 1:50,R&D Systems,MAB7619;

(3) Generation of human gastric assembloids from primary fetal organoidsPediatric Surgery InternationalNovember 24, 2023Giada Benedetti, Brendan C. Jones, Francesca Sgualdino, Paolo De Coppi, Giovanni Giuseppe Giobbemedium (Biorad, BUF058A). Primary antibodies: Chromogranin A (Abcam, ab15160), Mucin 5AC (Invitrogen, ma5-12178), Mucin 6 (Abcam, ab216017), Ki67 (Abcam, ab15580), Integrin beta-4 (INTB4) (Abcam, ab110167) all at

(4) Bei Mu Gua Lou San facilitates mucus expectoration by increasing surface area and hydration levels of airway mucus in an air-liquid-interface cell culture model of the respiratory epitheliumBMC Complementary Medicine and TherapiesNovember 17, 2023Silvia Groiss, Ina Somvilla, Christine Daxböck, Manuela Stückler, Elisabeth Pritz, Dagmar Brislingerantibodies MUC1,Ventana,790–4574,IHC,rtu MUC4,Ventana,406 M-16,IHC,1:30 MUC5AC,ThermoFisher Scientific,MA5-12178,"IHC, AlB-IF","1:500, 1:100" MUC5B,Invitrogen,PA5-82342,AlB-IF,1:100 PanCK,Ventana,MON5164-1,IHC,1:400 S100,Agilent

(5) Discovery of Fungus-Derived Nornidulin as a Novel TMEM16A Inhibitor: A Potential Therapy to Inhibit Mucus Secretion in AsthmaJournal of Experimental PharmacologyNovember 15, 2023Pawin Pongkorpsakol, Chantapol Yimnual, Wilasinee Satianrapapong, Nichakorn Worakajit, Suchada Kaewin, Praphatsorn Saetang, Vatcharin Rukachaisirikul, Chatchai MuanprasatTriton X before overnight incubation with mouse anti-MUC5AC monoclonal antibody (45M1) (catalog number: MA5-12178, Thermo Fisher Scientific Inc., Waltham, MA, USA). The fixed cells were washed five times with PBS and

(6) The Influenza B Virus Victoria and Yamagata Lineages Display Distinct Cell Tropism and Infection-Induced Host Gene Expression in Human Nasal Epithelial Cell CulturesVirusesSeptember 20, 2023Jo L. Wilson, Elgin Akin, Ruifeng Zhou, Anne Jedlicka, Amanda Dziedzic, Hsuan Liu, Katherine Z. J. Fenstermacher, Richard E. Rothman, Andrew PekoszMonoclonal,Primary,Invitrogen MA5-29901,1 μg/mL MUC5AC Monoclonal Antibody Clone: 45M1,Primary,Invitrogen MA5-12178,2 μg/mL "CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), PE, eBioscience",Conjugated-PE,Invitrogen

(7) A comparative study of in vitro air-liquid interface culture models of the human airway epithelium evaluating cellular heterogeneity and gene expression at single cell resolutionRespiratory researchAugust 28, 2023Prescott RA, Pankow AP, de Vries M, Crosse KM, Patel RS, Alu M, Loomis C, Torres V, Koralov S, Ivanova E, et al.α-MUC5B diluted 1:2000 (ThermoFisher, cat. no. PA5-82342), α-MUC5AC diluted 1:1000 (ThermoFisher, cat. no MA5-12178), α-p63 diluted 1:1250 (Abcam, cat. no. Ab124762), α-FoxJ1 diluted 1:300 (ThermoFisher, cat no. 14-9965-82),

(8) The Influenza B Virus Victoria and Yamagata Lineages Display Distinct Cell Tropism and Infection Induced Host Gene Expression in Human Nasal Epithelial Cell CulturesbioRxiv : the preprint server for biologyAugust 4, 2023Wilson JL, Akin E, Zhou R, Jedlicka A, Dziedzic A, Liu H, Fenstermacher KZ, Rothman R, Pekosz A.Monoclonal,Primary,Invitrogen MA5-29901,1 μg/mL MUC5AC Monoclonal Antibody Clone: 45M1,Primary,Invitrogen MA5-12178,2 μg/mL "CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), PE, eBioscience",Conjugated-PE,Invitrogen

(9) Growth media affects susceptibility of air-lifted human nasal epithelial cell cultures to SARS-CoV2, but not Influenza A, virus infectionbioRxiv : the preprint server for biologyAugust 2, 2023Resnick JD, Wilson JL, Anaya E, Conte A, Li M, Zhong W, Beer MA, Pekosz A.Anti-TMPRSS11E Clone TM191,Primary,392002,0.01 mg/mL MUC5AC Monoclonal Antibody Clone: 45M1,Primary,Invitrogen MA5–12178,2 μg/mL "CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), PE, eBioscience",Conjugated-PE,Invitrogen

(10) Full thickness 3D in vitro conjunctiva model enables goblet cell differentiationScientific ReportsJuly 28, 2023Julian Schwebler, Christina Fey, Daniel Kampik, Christian Lotz(Sigma-Aldrich, HPA023040, dilution 1:1000), Cytokeratin 19 (Abcam, ab7754, dilution 1:100), MUC5AC (Invitrogen, MA5-12178, dilution 1:100), Vimentin (Abcam, ab92547, dilution 1:2000), E-Cadherin (BD Biosciences, 610181, dilution

(11) A high-throughput cigarette smoke-treated bronchosphere model for disease-relevant phenotypic compound screeningPLOS ONEJune 29, 2023Pranjali Beri, Young Jae Woo, Katie Schierenbeck, Kaisheng Chen, S. Whitney Barnes, Olivia Ross, Douglas Krutil, Doug Quackenbush, Bin Fang, John Walker, et al.dilution 1:500) and incubated overnight at room temperature. Primary antibodies used were: MUC5AC (Thermo MA5-12178), MUC5B (Sigma HPA008246-100UL), α-acetylated tubulin (Sigma T6793-.2ML). Plates were washed three times

(12) Visualising SARS-CoV-2 infection of the lung in deceased COVID-19 patientsEBioMedicineJune 1, 2023Van Slambrouck J, Khan M, Verbeken E, Choi S, Geudens V, Vanluyten C, Feys S, Vanhulle E, Wollants E, Vermeire K, et al.1:200, Cytokeratin 8 (Novus Biologicals, Cat#NBP2-67468) at 1:100, MUC5AC (Thermo Fisher Scientific, Cat#MA5-12178) at 1:300, CD31 (Abcam, Cat#ab134168) at 1:100, Surfactant protein C (abbexa, Cat# abx027534-80) at

(13) Protocol to engineer apical-out airway organoids using suspension culture of human airway basal stem cell aggregatesSTAR ProtocolsJune 1, 2023Piyumi Wijesekara, Kian Z. Patel, Ellen L. Otto, Phil G. Campbell, Xi RenAnti-Acetylated-α-Tubulin,Sigma-Aldrich,Cat# T6793; RRID: AB_477585 Mouse Anti-MUC5AC,Thermo Fisher Scientific,Cat# MA5-12178; RRID: AB_10978001 Mouse Anti-FOXJ1,Thermo Fisher Scientific,Cat# 14-9965-82; RRID: AB_1548835 "Donkey

(14) A novel thiol-saccharide mucolytic for the treatment of muco-obstructive lung diseasesEuropean Respiratory JournalMay 25, 2023Annalisa Addante, Wilfred Raymond, Irina Gitlin, Annabelle Charbit, Xavier Orain, Aaron Wolfe Wolfe Scheffler, Aditi Kuppe, Julia Duerr, Maria Daniltchenko, Marika Drescher, et al.MUC5B (sc-393952; Santa Cruz, Dallas, TX, USA) and a mouse monoclonal antibody against human MUC5AC (MA5-12178; Invitrogen, Waltham, MA, USA) and for Western blots of BAL supernatants from mice, we used mouse monoclonal

(15) Primary Human Pancreatic Cancer Cells Cultivation in Microfluidic Hydrogel Microcapsules for Drug EvaluationAdvanced ScienceApril 1, 2023Taiyu Song, Hui Zhang, Zhiqiang Luo, Luoran Shang, Yuanjin Zhaosolution included MUC1 (ab109185, 1:250), Ki67 (27309‐1‐AP, 1:200), CD44 (ab254530, 1:250), MUC5AC (MA5‐12178, 1:100), CK19 (10712‐1‐AP, 1:200), and CD133 (AF06634, 1:200). Fluorescent‐labeled antibodies were antimouse

(16) Airway and Lung Organoids from Human-Induced Pluripotent Stem Cells Can Be Used to Assess CFTR ConductanceInternational journal of molecular sciencesMarch 27, 2023Demchenko A, Kondrateva E, Tabakov V, Efremova A, Salikhova D, Bukharova T, Goldshtein D, Balyasin M, Bulatenko N, Amelina E, et al.703809)",10 μg/mL "SCGB3A2 (Abcam, cat. no. ab181853)",26 μg/mL "MUC5AC (Thermo Fisher Scientific, cat. no. MA5-12178)",4 μg/mL "SOX9 (Thermo Fisher Scientific, cat. no. MA5-17177)",20 μg/mL "AQP1 (Sigma-Aldrich, cat.

(17) Cigarette smoke preferentially induces full length ACE2 expression in differentiated primary human airway cultures but does not alter the efficiency of cellular SARS-CoV-2 infectionHeliyonMarch 1, 2023Porter LM, Guo W, Crozier TW, Greenwood EJ, Ortmann B, Kottmann D, Nathan JA, Mahadeva R, Lehner PJ, McCaughan F.ACE2,Abcam,ab108209 Mouse Alpha-tubulin,Santa Cruz,sc-32293 Mouse acetylated tubulin,Sigma,T7451 Mouse Muc5AC,Invitrogen,MA5-12178 Mouse SARS-CoV-2 (COVID-19) spike antibody [1A9],Genetex,GTX632604 Mouse beta-actin,Santa Cruz,sc-69879 Bacterial

(18) A comparative study of in vitro air-liquid interface culture models of the human airway epithelium evaluating cellular heterogeneity and gene expression at single cell resolutionbioRxiv : the preprint server for biologyFebruary 28, 2023Prescott RA, Pankow AP, de Vries M, Crosse K, Patel RS, Alu M, Loomis C, Torres V, Koralov S, Ivanova E, et al.Cruz), α-MUC5B diluted 1:2000 (cat. no. PA5-82342, Thermo Fisher), and α-MUC5AC diluted 1:1000 (cat. no MA5-12178, Thermo Fisher). Slides were washed three times with wash buffer, then incubated in secondary antibody

(19) Inhibition of microRNA-328 Increases Ocular Mucin Expression and Conjunctival Goblet CellsBiomedicinesJanuary 19, 2023Jackson Choo, Chun-Huei Liao, Ching-Li Tseng, Jiunn-Liang Chen, Huey-Chuan Cheng, Chung-Ling Liang, Suh-Hang Hank Juoantibodies used in this study were confirmed to react with rabbit protein, including MUC5AC (Clone 45M1, MA5-12178, Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA) and beta-actin (ab8227, Abcam, Waltham,

(20) Nebulized Menthol Impairs Mucociliary Clearance via TRPM8 and MUC5AC/MUC5B in Primary Airway Epithelial CellsInternational journal of molecular sciencesJanuary 15, 2023Baumlin N, Silswal N, Dennis JS, Niloy AJ, Kim MD, Salathe M.0.05% Tween 20. A 3% BSA solution was used to block for 1 h before incubation with anti-MUC5AC (cat# MA512178; Thermo Fisher Scientific) and anti-MUC5B (cat# PA82342; Thermo Fisher Scientific) antibodies at 0.2

Be the First to Write a Review!

Cell stem cell

Cells

Nature communications

Disease models & mechanisms

Annals of translational medicine

MUC5AC Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

Citations (300)

Reviews

MUC5AC Antibody from Thermo Fisher Scientific

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map