Fig 2: SEPT6 mediated UBC expression. (a) The expression of SEPT6 was detected by qRT-PCR in prostate cancer tissues and paracancerous tissues. *P < 0.05 vs. paracancerous tissues. (b) Pearson correlation coefficient analyzed the relationship between SEPT6 and UBC in prostate cancer tissues. (c) IF detected the colocation of SEPT6 and UBC in prostate cancer cells. Scale bar = 25 µm, the magnification is 400 times. (d) The overexpression and knockdown efficiency of SEPT6 was detected by qRT-PCR. *P < 0.05 vs. the NC group. (e, f) qRT-PCR and WB detected UBC expression, respectively. *P < 0.05 vs. the NC group.

Fig 3: SEPT6 inhibited tumor growth in nude mice. (a) The tumor volume of each group was detected at the time points of 4, 7, 11, 14, 17, 21, 24, and 28 d. (b) Nude mice were sacrificed on day 28, and tumor mass was measured in each group. (c) UBC expressions were detected by qRT-PCR. (d) WB was performed to detect SEPT6, UBC, ubiquitin, PCNA, and Ki67 expressions. *P < 0.05 vs. the NC group.