Fig 1: Effects of Ang II and candesartan on the expressions of AT1R, PLC-β1 and CaM in HCC cells. A, B FCM assay was used to detect the expression of AT1R on the surface of HepG2 and HCCLM3 cells stimulated by Ang II and candesartan for 48 h. C, D Representative western blot images and semi-quantitative analysis showed the differences in PLC-β1 and CaM levels in HCC cells between different drug groups. *P < 0.05, **P < 0.01 VS control group
Fig 2: Western blot analysis of REN1, ACE1, AGTR1, AGTR2, CTGF, and COL3 in myocardial tissue in the in vivo and IVF groups at (A) 3 weeks, (B) 10 weeks, and (C) 1.5 years of age. GAPDH was used as the internal control. Data are presented as mean ± standard error of the mean. *P < 0.05 and **P < 0.01 compared with the in vivo group; Student t-test (N, in vivo = 4; IVF = 4 in 3-week-old mice; N, in vivo = 4; IVF = 4 in 10-week-old mice; and N, in vivo = 4; IVF = 4 in 1.5-year-old mice).
Fig 3: a Renal survival in stage 4 chronic kidney disease (CKD) patients with diabetic nephropathy. b Immunohistochemical staining for angiotensin II type 1 receptor (AT1R) expression in the glomeruli, renal tubules, and renal vessels of stage 4 CKD patients with diabetic nephropathy (DN) in the stable group and the progressive group (original magnification × 200). c Expression levels of AT1R in the glomeruli, renal tubules, and renal vessels of the stable group and the progressive group. AOD average optical density. *p < 0.05 versus stable group
Fig 4: AGTR1-related genes enrichment analysis. (A) The protein-protein interaction networks of experimentally determined AGTR1-binding proteins. (B) Heat map of the correlation between the top 10 AGTR1-related genes and AGTR1 in pan-cancer. All ten genes were significantly positively related to AGTR1 in lung adenocarcinoma (C) Pathway enrichment analysis of the AGTR1-related genes. (D–F) The top 10 items of GO analysis: biological processes, cellular components, and molecular functions of the AGTR1-related genes.
Fig 5: Correlation analysis between AGTR1 expression and PI3K/AKT, ERK1/2 pathways. (A) Heat map of the correlation between AGTR1 and the main molecules of PI3K/AKT, ERK1/2 signaling pathway. AGTR1 was positively correlated with AKT3 most PI3K gene subtypes. The KM analysis results of AKT3 (B), PIK3CD (C), PIK3CG (D), PIK3IP1 (E), PIK3R1 (F), PIK3R3 (G), PIK3R5 (H), PIK3R6 (I) in LUAD. Red represents high genes expression, blue represents low expression. (*P <0 0.05; **P < 0.01; ***P < 0.001).
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