Fig 1: Expression of candidate transcription factors in WT and arid4bΔ mESCs. Expression of ten candidate transcription factors (TFs) (Pdx1, Prrx2, Bach1, MafK, Znf354c, Mzf1, Ddit3, Tfap2c, Atoh1 and Stat6) were determined by RT-qPCR in a. wild-type (CJ9) b. arid4bΔ mESCs. ESC-specific TFs Nanog and Oct4 were used as positive controls whereas neuroectoderm-specific TF Sox1 was used as a negative control for comparison. Error bars represent standard error of three independent biological replicates. c. Expression of Bach1, Ddit3, Prrx2, Tfap2c and Znf354c were determined using Western blot analysis in WT and arid4bΔ mESCs. Actin was used as a loading control. The results are representative of three independent biological replicates. The quantification of Western blot replicates was performed using ImageJ. The values were first normalized to Actin and then represented as arid4bΔ over wild-type (WT). Error bars represent standard error. All values except Prrx2 (p-value < 0.005) were found nonsignificant in arid4bΔ vs. wild-type by unpaired t-test. d. Consensus DNA binding sequence representations of Bach1, Ddit3, Prrx2, Tfap2c and Znf354c (JASPAR).