Fig 2: CIM6P/IGF2R is required for learning-induced de novo protein synthesis in rats.SUnSET was employed to quantify de novo protein synthesis in the rat hippocampus. Puromycin was co-injected with IgG or anti-CIM6P/IGF2R prior to training, and rats were perfused 2 hr later. Upper panels: representative images of anti-puromycin immunostaining, composite tile scans of whole hippocampus (scale bar, 500 µm). Middle and lower panels: CA1 and DG (scale bar 10 µm). Bar graphs at right show immunostaining intensity quantifications for each sub-region (n = 4, two independent experiments). Two-way ANOVA followed by Tukey’s post hoc tests. **p<0.01, ***p<0.001; see Source data one for detailed statistical information.

Fig 3: CD-M6PR is essential for EV71 replication. (A) Western blotting of RAB5, RAB7, RAB9, CD-M6PR, CI-M6PR and clathrin heavy chain in cells in which these genes have been targeted by siRNA knockdown. The expression of ß-actin was examined as loading control. Triangles indicate molecular weight markers. Black dots indicate the predicted molecular weight of the proteins. The numbers are the densities and the ratio of anti-markers/anti ß-actin. (B) RD cells were treated with siRNAs targeting RAB5, RAB7, RAB9, CD-M6PR, CI-M6PR or clathrin heavy chain and then infected with EV71-GFP. Cells were fixed at 31 h after infection, followed by fluorescent imaging. GFP-positive cells were counted and their percentage against the total number of DAPI-positive cells plotted. *P<0.05. Statistical significance was determined by a two-tailed, unpaired t-test. Error bars represent s.d.

Fig 4: (a) Single-slice confocal image of CI-MPR (green) and CD31 (red) co-stain in cortex of healthy 3.9-year-old cynomolgus monkey. Scale bar = 5 µm. (b) CI-MPR (brown) IHC in the cortex of normal 3-month-old and 15-year-old human shows vascular and neuronal staining pattern. Low-res. image scale bar = 10 µm. High-res. image scale bar = 5 µm

Fig 5: ESCPE-1 knocksideways inactivates ESCPE-1 and results in a temporally resolved CI-MPR redistribution away from the TGN. (A) SNX-BAR knocksideways HeLa cells were fixed before or at multiple time points after the addition of rapalog and then labelled for Golgin97 and CI-MPR. The merged panel shows both the anti-Golgin97 and anti-CI-MPR channels with a magnified image (inset). (B) Pearson's colocalisation between Golgin97 and CI-MPR before or at multiple time points after the addition of rapalog. nexp=3, ncell=60 with all data points being displayed. (C) SNX-BAR knocksideways HeLa cells were fixed before or at multiple time points after the addition of rapalog and then labelled for LAMP1 and Itgß1. The merged panel shows both the LAMP1 and Itgß1 channels with a zoom panel. (D) Pearson's colocalisation between LAMP1 and Itgß1 before or at multiple time points after the addition of rapalog. nexp=3, ncell=60 with all data points being displayed. *P<0.05; ****P<0.0001; N/S, not significant (P>0.05) (ordinary one-way ANOVA with multiple comparisons). Error bars show the s.e.m. Scale bars: 10 µm.