Fig 1: (A) Detection of peak 1944 in the linear mode of MALDI-TOF-TOF. (B) Detection of peak 1944 in the reflection mode. (C) Detection of a fragment of peak 1944 in the lift mode of MALDI-TOF-TOF and matching the peptide. (D) MASCOT Search sore for KNG1. (E) Western blotting analysis of KNG1 in the serums of CRC, CRA and HC patients. (F) Western blotting analysis of KNG1 in the tissues of CRC(C1, C2, C3), CRA(A1, A2, A3) and adjacent normal(N1, N2, N3). ß-actin was used as loading control.
Fig 2: Western blot experiments were performed to determine the protein expression in eight corresponding normal (N) and clear cell renal cell carcinoma (T) tissue. NPTX2 was increased in tumor tissue, whereas FXYD4, KNG1, SLC12A1 and SLC6A3 were decreased in tumors. NDUF4AL2 levels were similar, but the bands were of different size in tumor and normal samples
Fig 3: The expression of 14 target mRNAs in renal cell carcinoma (red bars) and normal renal (green bars) tissue was validated using quantitative real-time PCR; the expression levels were normalized using ACTB and TBP as reference genes. Significant overexpression of SLC6A3, NPTX2, TFAIP6, NDUFA4L2, ENPP3, FABP6 and SPINK13 mRNA in renal cell carcinoma was confirmed, whereas FXYD4, SLC12A1, KNG1, NPHS2, SLC13A3, GCGR and PLG mRNA levels were downregulated (all p < 0.001)
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